Gdansk University of Technology, Chemical Faculty, Department of Microbiology, Gdańsk, Poland.
Gdansk University of Technology, Chemical Faculty, Department of Microbiology, Gdańsk, Poland.
Diagn Microbiol Infect Dis. 2014 Jul;79(3):342-6. doi: 10.1016/j.diagmicrobio.2014.03.005. Epub 2014 Mar 17.
The preliminary diagnostic utility of two mixtures of Toxoplasma gondii recombinant antigens (rROP1+rSAG2 and rROP1+rGRA6) in IgG ELISA and IgG avidity test has been evaluated. A total of 173 serum samples from patients with toxoplasmosis and seronegative people were examined. The sensitivity of IgG ELISA for rROP1+rSAG2 and rROP1+rGRA6 was 91.1% and 76.7%, respectively, while the reactivity for sera from patients where acute toxoplasmosis was suspected was higher, at 100% and 95.4%, respectively, than for people with chronic infection, at 88.2% and 70.6%. In this study a different trend in avidity maturation of IgG antibodies for two mixtures of proteins in comparison with native antigen was observed. The results suggest that a new IgG avidity test using the mixtures of recombinant antigens may be useful for the diagnosis of difficult-to-identify phases of toxoplasmosis. For this reason, selected mixtures after the additional tests on groups of sera with well-defined dates of infection could be used as a better alternative to the native antigens of the parasite in the serodiagnosis of human T. gondii infection.
我们评估了两种刚地弓形虫重组抗原（rROP1+rSAG2 和 rROP1+rGRA6）混合物在 IgG ELISA 和 IgG 亲和力试验中的初步诊断效用。共检测了 173 份来自弓形虫病患者和血清阴性人群的血清样本。rROP1+rSAG2 和 rROP1+rGRA6 的 IgG ELISA 敏感性分别为 91.1%和 76.7%，而对于怀疑急性弓形虫病的患者血清的反应性更高，分别为 100%和 95.4%，而对于慢性感染的患者，分别为 88.2%和 70.6%。在这项研究中，与天然抗原相比，两种蛋白质混合物的 IgG 抗体亲和力成熟呈现出不同的趋势。结果表明，使用重组抗原混合物的新 IgG 亲和力试验可能有助于诊断难以识别的弓形虫病阶段。因此，在对具有明确感染日期的血清组进行额外测试后，可以选择合适的混合物作为寄生虫天然抗原的替代物，用于人类刚地弓形虫感染的血清学诊断。
