Kuvakina V I, Alliluev A P, Basnak'ian I A, Valerius I I, Kotel'nikova O V
Zh Mikrobiol Epidemiol Immunobiol. 1986 Sep(9):3-7.
The optimum conditions for the isolation and purification of the specific polysaccharide of group B meningococci have been developed. The advantages of the use of synthetic culture media for growing the initial bacterial culture have been demonstrated. The purified polysaccharides have been found to contain about 70% of sialic acid and less than 1% of protein and nucleic acid admixtures. The molecular parameters of group B polysaccharide depended on the growth phase of the bacterial culture. The most valuable culture was obtained at the exponential phase of growth. High serological activity and specificity of the polysaccharide in the passive hemagglutination test recommend it for studies on the development of diagnostic and prophylactic preparations.
已确定了分离和纯化B群脑膜炎球菌特异性多糖的最佳条件。已证明使用合成培养基培养初始细菌培养物的优势。已发现纯化的多糖含有约70%的唾液酸以及少于1%的蛋白质和核酸混合物。B群多糖的分子参数取决于细菌培养物的生长阶段。在生长指数期可获得最有价值的培养物。该多糖在被动血凝试验中具有高血清学活性和特异性，推荐用于诊断和预防制剂开发的研究。
