Lu Wenwen, Wan Xinhua, Liu Bin, Rong Xianfang, Zhu Lei, Li Pingping, Li Jiang, Wang Ling, Cui Liying, Wang Xiaoliang
State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Department of Pharmacology, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.
Department of Neurology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.
PLoS One. 2014 Apr 25;9(4):e95684. doi: 10.1371/journal.pone.0095684. eCollection 2014.
The aim of this study is to identify and validate protein change in the serum from PD patients. We used serum samples from 21 PD patients and 20 age-matched normal people as control to conduct a comparative proteomic study. We performed 2-DE and analyzed the differentially expressed protein spots by LC-MS/MS. In PD group 13 spots were shown to be differentially expressed compared to control group. They were identified as 6 proteins. Among these, 3 proteins were confirmed by Western blot analysis. It showed that the frequency of fibrinogen γ-chain (FGG) appeared 70% in PD, which could not be detected in control group. The protein of inter-alpha-trypsin inhibitor heavy chain H4 (ITI-H4) was found to exist two forms in serum. The full size (120 kDa) of the protein was increased and the fragmented ITI-H4 (35 kDa) was decreased in PD group. The ratio of full size ITI-H4 to fragmented ITI-H4 in PD patients was 3.85 ± 0.29-fold higher than in control group. Furthermore, fragmented Apo A-IV (∼ 26 kDa) was mainly detected in control group, while it was rare to be found in PD group. Above findings might be useful for diagnosis of PD. When the expressions of FGG and 120 kDa ITI-H4 are increase, as well as ∼ 26 kDa Apo A-IV disappear would provide strong evidence for PD.
本研究的目的是识别和验证帕金森病（PD）患者血清中的蛋白质变化。我们使用了21例PD患者的血清样本以及20例年龄匹配的正常人作为对照，进行了一项比较蛋白质组学研究。我们进行了二维电泳（2-DE），并通过液相色谱-串联质谱（LC-MS/MS）分析差异表达的蛋白质斑点。在PD组中，与对照组相比有13个斑点显示出差异表达。它们被鉴定为6种蛋白质。其中，3种蛋白质通过蛋白质免疫印迹分析得到了证实。结果显示，纤维蛋白原γ链（FGG）在PD患者中的出现频率为70%，而在对照组中未检测到。发现α-胰蛋白酶抑制剂重链H4（ITI-H4）蛋白在血清中存在两种形式。在PD组中，该蛋白的全长（120 kDa）增加，而片段化的ITI-H4（35 kDa）减少。PD患者中全长ITI-H4与片段化ITI-H4的比例比对照组高3.85±0.29倍。此外，片段化的载脂蛋白A-IV（约26 kDa）主要在对照组中检测到，而在PD组中很少发现。上述发现可能对PD的诊断有用。当FGG和120 kDa ITI-H4的表达增加，以及约26 kDa载脂蛋白A-IV消失时，将为PD提供有力证据。
