Nobes C D, Lakin-Thomas P L, Brand M D
Department of Biochemistry, University of Cambridge, U.K.
Biochim Biophys Acta. 1989 Sep 28;976(2-3):241-5. doi: 10.1016/s0005-2728(89)80236-1.
In less than 1 min ouabain maximally inhibits oxygen consumption due to gramicidin-induced ATP turnover by the Na+/K+-ATPase in hepatocytes. Ouabain rapidly inhibits respiration on palmitate or glucose by only 6-10% indicating that the Na+/K+-ATPase plays a minor role in cell ATP turnover. 29% of the extra oxygen consumption of hepatocytes isolated from hyperthyroid rats was inhibited by ouabain showing that the Na+/K+-ATPase is responsible for some but not the majority of the stimulation of respiration induced by thyroid hormone.
在不到1分钟的时间内，哇巴因就能最大程度地抑制肝细胞中短杆菌肽诱导的钠钾ATP酶引起的ATP周转所导致的氧气消耗。哇巴因仅使棕榈酸酯或葡萄糖的呼吸作用迅速抑制6%-10%，这表明钠钾ATP酶在细胞ATP周转中起次要作用。从甲状腺功能亢进大鼠分离出的肝细胞，其额外氧气消耗的29%被哇巴因抑制，这表明钠钾ATP酶对甲状腺激素诱导的呼吸刺激有一定作用，但不是主要作用。
