Bergqvist Y, Karlsson L, Fohlin L
Department of Clinical Chemistry, Falun Central Hospital, Sweden.
Clin Chem. 1989 Oct;35(10):2127-9.
This simple method of centrifugal analysis for total protein in human breast milk is based on the change in the wavelength of the absorbance maximum of Coomassie Brilliant Blue G-250 when the dye is bound to protein. Within-run and between-day CVs were 3.8% and 4.8%, respectively. Compared with a micro-Kjeldahl method for determination of total nitrogen, the coefficient of correlation was 0.99.
这种用于分析人母乳中总蛋白质的简单离心分析方法基于考马斯亮蓝G-250染料与蛋白质结合时最大吸光度波长的变化。批内和批间变异系数分别为3.8%和4.8%。与微量凯氏定氮法测定总氮相比，相关系数为0.99。
