Graduate Program in Health Science, Federal University of Sergipe, Aracaju, SE, Brazil.
Graduate Program in Physiological Science, Federal University of Sergipe, Aracaju, SE, Brazil.
Evid Based Complement Alternat Med. 2014;2014:485043. doi: 10.1155/2014/485043. Epub 2014 Mar 19.
Background. Injections of acidic saline into the gastrocnemius muscle in rats produce a bilateral long-lasting hyperalgesia similar to fibromyalgia in humans. No previous study investigated the effect of electroacupuncture (EA) on this acidic saline model. This study aimed to identify the effects of EA in the hyperalgesia produced by repeated intramuscular injections of acidic saline. Methods. Rats were divided into four groups (n = 6, each group): control, acupuncture, EA 15 Hz, and 100 Hz. Left gastrocnemius muscle was injected with 100 μ L of pH 4.0 sterile saline twice five days apart. EA, acupuncture, or control therapy was daily administered (20 min) for 5 consecutive days under anesthesia. Needles were placed in the St36 and Sp6 acupoints. The assessment of secondary mechanical hyperalgesia, thermal hyperalgesia, and motor performance was performed before injections and before and after the treatment performed on each day. The paw withdrawal threshold was tested using the nonparametric Kruskal-Wallis test and differences within the group Wilcoxon Matched Pairs. The latency and motor performance were tested for ANOVA parametric test for independent measures, and for differences in the group, we used t-test for paired samples. Post hoc Tukey test was used for multiple corrections. P values less than 0.05 were considered statistically significant. Results. Indicate that there was a significant reduction of mechanical withdrawal threshold and paw withdrawal latency 24 hours following the second injection. Moreover, mechanical and thermal hyperalgesia were significantly reversed by EA 15, 100 Hz, and acupuncture. Conclusions. The results suggest that EA high and low frequency as well as acupuncture are effective in reducing hyperalgesia in chronic muscle pain model.
向大鼠的腓肠肌内注射酸性生理盐水会产生类似于人类纤维肌痛的双侧长期痛觉过敏。以前没有研究调查过电针对这种酸性生理盐水模型的影响。本研究旨在确定电针对反复肌肉内注射酸性生理盐水引起的痛觉过敏的影响。
将大鼠分为四组（每组 6 只）：对照组、针刺组、15Hz 电针组和 100Hz 电针组。左腓肠肌每隔五天注射 100 μ L pH4.0 无菌生理盐水两次。在麻醉下，每天进行电针、针刺或对照治疗（20 分钟），连续 5 天。将针放置在 St36 和 Sp6 穴位上。在注射前、治疗前和治疗后，评估二次机械性痛觉过敏、热痛觉过敏和运动表现。使用非参数 Kruskal-Wallis 检验测试足底退缩阈值，并对每组内的差异进行 Wilcoxon 配对样本检验。潜伏期和运动表现使用独立样本的方差参数检验进行测试，对于组间差异，我们使用配对样本 t 检验。使用事后 Tukey 检验进行多重校正。P 值小于 0.05 被认为具有统计学意义。
表明第二次注射后 24 小时，机械性退缩阈值和足底退缩潜伏期明显降低。此外，电针 15Hz、100Hz 和针刺均能显著逆转机械性和热痛觉过敏。
结果表明，电针高频和低频以及针刺均能有效减轻慢性肌肉疼痛模型中的痛觉过敏。
