Park Sang-Yong, Hwang Eunson, Shin Yu-Kyong, Lee Don-Gil, Yang Jung-Eun, Park Jae-Hee, Yi Tae-Hoo
Department of Oriental Medicinal Materials & Processing, College of Life Science, Kyung Hee University, Yongin-si, South Korea.
Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Yongin-Si, South Korea.
Mar Biotechnol (NY). 2017 Feb;19(1):65-75. doi: 10.1007/s10126-017-9727-y. Epub 2017 Jan 30.
Hizikia fusiforme, a brown seaweed, has been utilized as a health food and in traditional medicine. In this study, we investigated whether enzyme-modified H. fusiforme extracts (EH) have immunological effects compared with normal H. fusiforme extracts (NH). The effects of NH and EH on immune responses were investigated by assessing nitric oxide (NO) production, phagocytosis, and cytokine secretion in RAW 264.7 murine macrophages and mice. Also, fucosterol was evaluated to find the active component of NH and EH by addressing cytotoxicity test and NO production. Both of NH and EH significantly increased cell viability and NO synthesis. Tumor necrosis factor-α (TNF-α) expression was more induced by EH with LPS treatment. Phagocytic activity, as the primary function of macrophages, was markedly induced by EH treatment. Additionally, EH encouraged splenocyte proliferation and recovered the levels of cytokines IL-1β, IL-6, and TNF-α in mice. Finally, fucosterol increased NO production with no cytotoxicity, which means that fucosterol is an active component of EH. In conclusion, EH has the potential to modulate immune function and could offer positive therapeutic effect for immune system diseases.
羊栖菜，一种褐藻，已被用作保健食品和传统药物。在本研究中，我们调查了酶改性羊栖菜提取物（EH）与普通羊栖菜提取物（NH）相比是否具有免疫作用。通过评估RAW 264.7小鼠巨噬细胞和小鼠中一氧化氮（NO）的产生、吞噬作用和细胞因子分泌，研究了NH和EH对免疫反应的影响。此外，通过细胞毒性试验和NO产生评估，对岩藻甾醇进行评估以找出NH和EH的活性成分。NH和EH均显著提高细胞活力和NO合成。在LPS处理下，EH对肿瘤坏死因子-α（TNF-α）表达的诱导作用更强。作为巨噬细胞的主要功能，吞噬活性在EH处理后明显增强。此外，EH促进脾细胞增殖，并恢复小鼠体内细胞因子IL-1β、IL-6和TNF-α的水平。最后，岩藻甾醇增加NO产生且无细胞毒性，这意味着岩藻甾醇是EH的活性成分。总之，EH具有调节免疫功能的潜力，可为免疫系统疾病提供积极的治疗效果。
