Basic Research Laboratory, Department of Semiconductor Science and Technology, Chonbuk National University , Jeonju 561-756, Republic of Korea.
Anal Chem. 2014 Jun 3;86(11):5330-7. doi: 10.1021/ac5001916. Epub 2014 May 22.
Despite significant improvement in separation efficiency using nanostructure-based platforms, the mechanism underlying the high efficiency of rare cell capture remains elusive. Here we report on the first mechanistic study by developing highly controlled nanostructures to investigate cell surface nanomorphology to better understand the cellular response of CD4(+) T-lymphocytes in contact with nanostructured surfaces and to elucidate key mechanisms for enhancing separation efficiency. Our results showed that actin-rich filopodia protruded from T-cells in the early stage of cell capture (<20 min), demonstrate the different morphologies in response to various quartz nanopillar (QNP) arrays functionalized with streptavidin and the generation of sufficient adhesion sites for rendering more stable binding through three-dimensional local nanotopographic interactions between filopodia-QNPs and cell-substrate, leading to synergistic effects for enhancing cell-capture efficiency. This responsive mechanism of T-cells on nanotopographic templates provides new insights to understand the enhanced cell-capture efficiency and specificity from the primary cell suspension on nanostructured substrates.
尽管基于纳米结构的平台在提高分离效率方面取得了重大进展，但高纯度稀有细胞捕获的高效机制仍难以捉摸。在这里，我们通过开发高度可控的纳米结构来研究细胞表面纳米形态，首次对其进行了机制研究，以更好地理解 CD4(+)T 淋巴细胞与纳米结构表面接触时的细胞反应，并阐明提高分离效率的关键机制。我们的结果表明，在细胞捕获的早期（<20 分钟），富含肌动蛋白的丝状伪足从 T 细胞中伸出，证明了 T 细胞对不同功能化有链霉亲和素的石英纳柱（QNP）阵列的不同形态反应，以及通过丝状伪足-QNP 和细胞-基底之间的三维局部纳米形貌相互作用产生足够的粘附位点，从而通过协同作用提高细胞捕获效率。T 细胞对纳米形貌模板的这种响应机制为理解从纳米结构基底上的原始细胞悬浮液中增强的细胞捕获效率和特异性提供了新的见解。
