Department of Physics, Chung-Ang University, Seoul, 06974, Republic of Korea.
Department of Chemistry, College of Science and Arts, and Promising Centre for Sensors and Electronic Devices (PCSED), Najran University, Najran, 11001, Saudi Arabia.
Sci Rep. 2017 Nov 2;7(1):14917. doi: 10.1038/s41598-017-14884-x.
The detection of cancer biomarkers has recently attracted significant attention as a means of determining the correct course of treatment with targeted therapeutics. However, because the concentration of these biomarkers in blood is usually relatively low, highly sensitive biosensors for fluorescence imaging and precise detection are needed. In this study, we have successfully developed vertical GaN micropillar (MP) based biosensors for fluorescence sensing and quantitative measurement of CA15-3 antigens. The highly ordered vertical GaN MP arrays result in the successful immobilization of CA15-3 antigens on each feature of the arrays, thereby allowing the detection of an individual fluorescence signal from the top surface of the arrays owing to the high regularity of fluorophore-tagged MP spots and relatively low background signal. Therefore, our fluorescence-labeled and CA15-3 functionalized vertical GaN-MP-based biosensor is suitable for the selective quantitative analysis of secreted CA15-3 antigens from MCF-7 cell lines, and helps in the early diagnosis and prognosis of serious diseases as well as the monitoring of the therapeutic response of breast cancer patients.
近年来，癌症生物标志物的检测作为一种确定靶向治疗正确治疗方案的手段引起了广泛关注。然而，由于这些生物标志物在血液中的浓度通常相对较低，因此需要高灵敏度的荧光成像生物传感器和精确的检测方法。在这项研究中，我们成功地开发了基于垂直 GaN 微柱（MP）的荧光传感和 CA15-3 抗原定量测量的生物传感器。高度有序的垂直 GaN MP 阵列可成功地将 CA15-3 抗原固定在阵列的每个特征上，从而可以从阵列的顶表面检测到单个荧光信号，这是由于荧光标记的 MP 点的高规则性和相对较低的背景信号。因此，我们的荧光标记和 CA15-3 功能化的基于垂直 GaN-MP 的生物传感器适用于 MCF-7 细胞系中分泌的 CA15-3 抗原的选择性定量分析，有助于严重疾病的早期诊断和预后以及乳腺癌患者的治疗反应监测。
