Zheng Zhi, Kilian Andrzej, Yan Guijun, Liu Chunji
Commenwealth Scientific and Industrial Research Organisation (CSIRO) Plant Industry, St. Lucia, Queensland, Australia; School of Plant Biology, Faculty of Science and UWA Institute of Agriculture, The University of Western Australia, Perth, Australia; National Foxtail Millet Improvement Centre, Institute of Millet Crops, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang, China.
Diversity Arrays Technology Pty Ltd, Yarralumla, Canberra, Australia.
PLoS One. 2014 Apr 28;9(4):e96011. doi: 10.1371/journal.pone.0096011. eCollection 2014.
Fusarium crown rot (FCR) is one of the most damaging cereal diseases in semi-arid regions worldwide. The genetics of FCR resistance in the bread wheat (Triticum eastivum L.) variety EGA Wylie, the most resistant commercial variety available, was studied by QTL mapping. Three populations of recombinant inbred lines were developed with this elite variety as the resistant parent. Four QTL conferring FCR resistance were detected and resistance alleles of all of them were derived from the resistant parent EGA Wylie. One of these loci was located on the short arm of chromosome 5D (designated as Qcrs.cpi-5D). This QTL explains up to 31.1% of the phenotypic variance with an LOD value of 9.6. The second locus was located on the long arm of chromosome 2D (designated as Qcrs.cpi-2D) and explained up to 20.2% of the phenotypic variance with an LOD value of 4.5. Significant effects of both Qcrs.cpi-5D and Qcrs.cpi-2D were detected in each of the three populations assessed. Another two QTL (designated as Qcrs.cpi-4B.1 and Qcrs.cpi-4B.2, respectively) were located on the short arm of chromosome 4B. These two QTL explained up to 16.9% and 18.8% of phenotypic variance, respectively. However, significant effects of Qcrs.cpi-4B.1 and Qcrs.cpi-4B.2 were not detected when the effects of plant height was accounted for by covariance analysis. The elite characteristics of this commercial variety should facilitate the incorporation of the resistance loci it contains into breeding programs.
镰刀菌冠腐病（FCR）是全球半干旱地区最具破坏性的谷物病害之一。通过QTL定位研究了面包小麦（Triticum eastivum L.）品种EGA Wylie（现有抗性最强的商业品种）对FCR的抗性遗传。以这个优良品种为抗性亲本，构建了三个重组自交系群体。检测到四个赋予FCR抗性的QTL，它们的抗性等位基因均来自抗性亲本EGA Wylie。其中一个位点位于5D染色体短臂上（命名为Qcrs.cpi - 5D）。该QTL解释了高达31.1%的表型变异，LOD值为9.6。第二个位点位于2D染色体长臂上（命名为Qcrs.cpi - 2D），解释了高达20.2%的表型变异，LOD值为4.5。在评估的三个群体中均检测到Qcrs.cpi - 5D和Qcrs.cpi - 2D的显著效应。另外两个QTL（分别命名为Qcrs.cpi - 4B.1和Qcrs.cpi - 4B.2）位于4B染色体短臂上。这两个QTL分别解释了高达16.9%和18.8%的表型变异。然而，在通过协方差分析考虑株高效应时，未检测到Qcrs.cpi - 4B.1和Qcrs.cpi - 4B.2的显著效应。这个商业品种的优良特性应有助于将其所含的抗性位点纳入育种计划。
