CSIRO Plant Industry, 306 Carmody Road, St Lucia, QLD, 4067, Australia.
Theor Appl Genet. 2013 Oct;126(10):2511-20. doi: 10.1007/s00122-013-2151-4. Epub 2013 Jul 4.
Fusarium crown rot (FCR) is a serious cereal disease in semi-arid regions worldwide. In assisting the effort of breeding cultivars with enhanced resistance, we identified several barley genotypes with high levels of FCR resistance. One of these genotypes, AWCS079 which is a barley landrace originating from Japan, was investigated by developing and assessing three populations of recombinant inbred lines. Two QTL, one located on the long arm of chromosome 1H (designated as Qcrs.cpi-1H) and the other on 3HL (designated as Qcrs.cpi-3H), were found to be responsible for the FCR resistance of this genotype. Qcrs.cpi-1H is novel as no other FCR loci have been reported on this chromosome arm. Qcrs.cpi-3H co-located with a reduced height (Rht) locus and the effectiveness of the former was significantly affected by the latter. The total phenotypic variance explained by these two QTL was over 60 %. Significant effects were detected for each of the QTL in each of the three populations assessed. The existence of these loci with major effects should not only facilitate breeding and exploitation of FCR-resistant barley cultivars but also their further characterization based on fine mapping and map-based gene cloning.
镰刀菌顶腐病（FCR）是全球半干旱地区一种严重的谷类病害。为了帮助培育具有增强抗性的品种，我们鉴定出了几种具有高 FCR 抗性的大麦基因型。其中一个基因型 AWCS079 是一种源自日本的大麦地方品种，通过开发和评估三种重组自交系群体对其进行了研究。发现有两个 QTL 负责该基因型的 FCR 抗性，一个位于 1H 染色体的长臂上（命名为 Qcrs.cpi-1H），另一个位于 3HL 上（命名为 Qcrs.cpi-3H）。Qcrs.cpi-1H 是新颖的，因为在该染色体臂上没有报道过其他 FCR 基因座。Qcrs.cpi-3H 与矮秆（Rht）基因座共定位，前者的有效性受后者显著影响。这两个 QTL 总共解释了超过 60%的表型方差。在所评估的三个群体中的每一个群体中，都检测到了这两个 QTL 的显著效应。这些具有主要效应的基因座的存在不仅应该有助于培育和利用抗 FCR 的大麦品种，而且还应该有助于根据精细图谱和图谱基因克隆对它们进行进一步的特征描述。
