Yang Xia, Zhang Leilei, Wei Jiajie, Liu Lexin, Liu Di, Yan Xiangning, Yuan Minjie, Zhang Lingran, Zhang Ning, Ren Yan, Chen Feng
Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping /Agronomy College, Henan Agricultural University, Zhengzhou, China.
Nat Commun. 2025 Mar 15;16(1):2549. doi: 10.1038/s41467-025-57936-x.
Fusarium crown rot (FCR) is a serious underlying disease to threaten wheat yield and quality recently. Here, we identify a catalase antioxidant enzyme (TaCAT2) through genome wide association study (GWAS) and whole-exome sequencing (WES) in two nested bi-parental populations. We verify the function of TaCAT2 regulating wheat FCR resistance by genetic transformation. Moreover, we screen a sucrose non-fermenting-1-related protein kinase alpha subunit (TaSnRK1α) interacting with TaCAT2, and subsequently find that TaSnRK1α phosphorylates TaCAT2. We next identify an FCR-resistance haplotype TaCAT2, and confirm that Ser214 of TaCAT2 is a key phosphorylation site for TaSnRK1α. We also find that TaSnRK1α results in higher protein accumulation in TaCAT2 than in TaCAT2, which possibly contribute to scavenging ROS (reactive oxygen species) in TaCAT2 wheat plants. Furthermore, the function of TaSnRK1α regulating FCR resistance is verified by genetic transformation. Taken together, we propose a TaSnRK1α-TaCAT2 model to mediate FCR resistance by scavenging the ROS in wheat plants.
镰刀菌冠腐病（FCR）是近年来威胁小麦产量和品质的一种严重潜在病害。在此，我们通过全基因组关联研究（GWAS）和全外显子组测序（WES）在两个嵌套的双亲亲本群体中鉴定出一种过氧化氢酶抗氧化酶（TaCAT2）。我们通过遗传转化验证了TaCAT2调节小麦对FCR抗性的功能。此外，我们筛选出一种与TaCAT2相互作用的蔗糖非发酵-1相关蛋白激酶α亚基（TaSnRK1α），随后发现TaSnRK1α使TaCAT2磷酸化。接下来，我们鉴定出一种FCR抗性单倍型TaCAT2，并证实TaCAT2的Ser214是TaSnRK1α的关键磷酸化位点。我们还发现TaSnRK1α导致TaCAT2中的蛋白质积累高于TaCAT2，这可能有助于清除TaCAT2小麦植株中的活性氧（ROS）。此外，通过遗传转化验证了TaSnRK1α调节FCR抗性的功能。综上所述，我们提出了一个TaSnRK1α-TaCAT2模型，通过清除小麦植株中的ROS来介导FCR抗性。
