基于纳米多孔氧化铝膜的阻抗型 DNA 生物传感器用于检测登革热病毒的特定寡核苷酸序列。

Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, Singapore 637371, Singapore.

Sensors (Basel). 2013 Jun 17;13(6):7774-85. doi: 10.3390/s130607774.

A novel and integrated membrane sensing platform for DNA detection is developed based on an anodic aluminum oxide (AAO) membrane. Platinum electrodes (~50-100 nm thick) are coated directly on both sides of the alumina membrane to eliminate the solution resistance outside the nanopores. The electrochemical impedance technique is employed to monitor the impedance changes within the nanopores upon DNA binding. Pore resistance (Rp) linearly increases in response towards the increasing concentration of the target DNA in the range of 1 × 10⁻¹² to 1 × 10⁻⁶ M. Moreover, the biosensor selectively differentiates the complementary sequence from single base mismatched (MM-1) strands and non-complementary strands. This study reveals a simple, selective and sensitive method to fabricate a label-free DNA biosensor.

一种基于阳极氧化铝（AAO）膜的新型集成膜传感平台被开发用于 DNA 检测。铂电极（~50-100nm 厚）直接涂覆在氧化铝膜的两侧，以消除纳米孔外的溶液电阻。电化学阻抗技术被用于监测 DNA 结合时纳米孔内的阻抗变化。孔电阻（Rp）随目标 DNA 浓度在 1×10⁻¹² 到 1×10⁻⁶ M 的范围内呈线性增加。此外，该生物传感器能够选择性地区分互补序列与单碱基错配（MM-1）链和非互补链。本研究揭示了一种简单、选择性和灵敏的方法来制备无标记的 DNA 生物传感器。