Khalilak Zohreh, Vatanpour Mehdi, Javidi Maryam, Mafi Moravrid, Afkhami Farzaneh, Daneshvar Farzaneh
Department of Endodontics, Dental School, Islamic Azad University of Medical Science, Tehran, Iran.
Department of Endodontics, Dental Material Research Center/Dental School, Mashad University of Medical Sciences, Mashad, Iran.
Iran Endod J. 2011 Spring;6(2):60-4. Epub 2011 May 15.
Blood contamination of the canal during preparation can be a major problem in endodontics; this may result in apical microleakage. The aim of this in vitro study was to evaluate the effect of blood on apical microleakage of a resin-based root canal sealer (AH26) and a polymer-based root canal sealer (Epiphany).
In this experimental study, 50 decoronated central incisors and canine teeth were prepared by RaCe rotary system and randomly divided into 4 groups (n=10). Groups A1 and A2 were obturated by Epiphany/Resilon and AH26/Gutta-percha, respectively. The obturations were performed with a single cone technique after drying root canals. In B1 and B2 groups, the test groups, 0.02cc citrated human blood was injected into dried root canals and they were obturated in the same manner. Ten specimens were served as positive and negative controls (n=5).The apical leakage was measured by means of a computerized fluid filtration method after 1 day and 3 weeks. The data was analyzed by One-Sample Kolmogorov-Smirnov, Independent Sample t-test and univariate analysis. Statistical significances were preset at α=0.05.
There was no significant difference in apical microleakage of the two sealers after 1 day and 3 weeks in dry and blood environment (P>0.05). Sealer and environment had no interaction (P>0.05).
Blood contamination has no significant effect on the apical microleakage of Epiphny and AH26.
根管预备过程中根管内的血液污染可能是牙髓病学中的一个主要问题；这可能导致根尖微渗漏。本体外研究的目的是评估血液对树脂基根管封闭剂（AH26）和聚合物基根管封闭剂（Epiphany）根尖微渗漏的影响。
在本实验研究中，用RaCe旋转系统对50颗去除冠部的中切牙和尖牙进行预备，并随机分为4组（n = 10）。A1组和A2组分别用Epiphany/Resilon和AH26/牙胶进行充填。根管干燥后采用单尖技术进行充填。在B1组和B2组（测试组）中，将0.02cc枸橼酸化人血注入干燥的根管中，并以相同方式进行充填。10个标本作为阳性和阴性对照（n = 5）。1天和3周后通过计算机化流体过滤法测量根尖渗漏。数据采用单样本柯尔莫哥洛夫-斯米尔诺夫检验、独立样本t检验和单因素分析进行分析。统计学显著性预设为α = 0.05。
在干燥和有血液的环境中，1天和3周后两种封闭剂的根尖微渗漏无显著差异（P>0.05）。封闭剂与环境之间无相互作用（P>0.05）。
血液污染对Epiphny和AH26的根尖微渗漏无显著影响。
