Mulyawati Ema, Soesatyo Marsetyawan Hne, Sunarintyas Siti, Handajani Juni
Department of Conservative Dentistry, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Faculty of Medicine, Public Health and Nursing, Yogyakarta, Indonesia.
Contemp Clin Dent. 2020 Apr-Jun;11(2):136-140. doi: 10.4103/ccd.ccd_447_19. Epub 2020 Aug 7.
The success of root canal treatment is influenced by hermetic root canal obturation. This study was conducted to analyze the apical sealing ability after the addition of calcite-synthesized hydroxyapatite (HA) as an epoxy resin sealer filler.
Calcite-synthesized HA powder was prepared using the microwave hydrothermal process. HA resin sealer powder and epoxy resin paste (3:1) were mixed, and concentrations of 10%, 20%, 30%, 40%, and 50% were prepared. A sample of thirty maxillary incisors were prepared in the root canal and then, the crown was cut to leave 13 mm of the root and a working length of 12 mm. The root canal was prepared using the crown-down technique and irrigated using 2.5% sodium hypochlorite and 17% ethylenediaminetetraacetic acid alternately. The samples were divided into six groups, with each group consisting of five roots. Group I was obturated with gutta percha using an epoxy resin sealer without HA (HA-0%) as a control group. In each of the Groups II, III, IV, V, and VI, 10% HA resin sealer, 20% HA, 30% HA, 40% HA, and 50% HA were used. All the samples were incubated in a 10-ml simulated body fluid solution at 37°C for 4 weeks. Apical closure density measurement was done using a scanning electron microscope, and the results were analyzed using the Kruskal-Wallis and Mann-Whitney U-tests.
A significant increase in the apical sealing ability was observed in the HA-20% sealer group and the HA-30% and HA-40% groups compared to that in the control group. However, the HA-50% sealer group showed a decrease in the apical sealing ability, whereas the HA-10% sealer group showed no difference. The HA-30% had the highest sealing ability than other concentrations.
The addition of calcite-synthesized HA as a filler at concentrations of 20%, 30%, and 40% increased the apical sealing ability of the epoxy resin sealer.
根管治疗的成功受到根管严密封闭的影响。本研究旨在分析添加方解石合成羟基磷灰石（HA）作为环氧树脂封闭剂填料后的根尖封闭能力。
采用微波水热法制备方解石合成HA粉末。将HA树脂封闭剂粉末与环氧树脂糊剂（3:1）混合，制备10%、20%、30%、40%和50%的浓度。选取30颗上颌切牙制备根管，然后截冠，保留13mm牙根，工作长度为12mm。采用冠向下技术预备根管，交替使用2.5%次氯酸钠和17%乙二胺四乙酸冲洗。将样本分为六组，每组五根牙根。第一组用不含HA的环氧树脂封闭剂（HA-0%）充填牙胶作为对照组。第二组、第三组、第四组、第五组和第六组分别使用10% HA树脂封闭剂、20% HA、30% HA、40% HA和50% HA。所有样本在37℃的10ml模拟体液溶液中孵育4周。使用扫描电子显微镜进行根尖封闭密度测量，并采用Kruskal-Wallis检验和Mann-Whitney U检验分析结果。
与对照组相比，HA-20%封闭剂组、HA-30%组和HA-40%组的根尖封闭能力显著提高。然而，HA-50%封闭剂组的根尖封闭能力下降，而HA-10%封闭剂组无差异。HA-30%的封闭能力高于其他浓度组。
添加20%、30%和40%浓度的方解石合成HA作为填料可提高环氧树脂封闭剂的根尖封闭能力。
