西西里岛农民贾第虫和隐孢子虫职业暴露的研究。
A study on occupational exposure of Sicilian farmers to Giardia and Cryptosporidium.
作者信息
Di Piazza F, Di Benedetto M A, Maida C M, Glorioso S, Adamo G, Mazzola T, Firenze A
出版信息
J Prev Med Hyg. 2013 Dec;54(4):212-7.
INTRODUCTION
A cross-sectional study was undertaken to determine the prevalence of Giardia and Cryptosporidium in calves of Palermo area (Sicily) and to evaluate the occupational risk associated with occurrence of zoonotic genotypes.
METHODS
A total of 217 faecal samples, from 149 calves (between 2 and 240 days of age) and 68 farmers, were collected in 19 cattle-farms of Palermo area. A questionnaire regarding demographic characteristics and personal hygienic measures was submitted to all farmers. All faecal samples were analyzed by Immunofluorescence assay and Polimerase Chain Reaction (PCR); genotypes were determined by DNA sequencing of Triose Phosphate Isomerase gene for Giardia and Small Subunit Ribosomal RNA gene for Cryptosporidium.
RESULTS
None farmer tested was positive for Giardia and Cryptosporidium, whereas these protozoa were respectively detected in 53 (including 5 with zoonotic G. duodenalis genotype A) and 17 (of which 1 with zoonotic C. ubiquitum) of the examined calves.
DISCUSSION
The results indicate that the risk of transmitting both protozoa to farmers in Palermo area is negligible although it cannot be considered null because of identification of human genotypes/species in calves.
引言
开展了一项横断面研究，以确定巴勒莫地区（西西里岛）犊牛中贾第虫和隐孢子虫的流行情况，并评估与动物基因型出现相关的职业风险。
方法
在巴勒莫地区的19个养牛场收集了总共217份粪便样本，其中包括149头犊牛（年龄在2至240天之间）和68位农民的样本。向所有农民发放了一份关于人口统计学特征和个人卫生措施的问卷。所有粪便样本均通过免疫荧光测定法和聚合酶链反应（PCR）进行分析；通过对贾第虫的磷酸丙糖异构酶基因和隐孢子虫的小亚基核糖体RNA基因进行DNA测序来确定基因型。
结果
所检测的农民中没有一人贾第虫和隐孢子虫呈阳性，而在所检查的犊牛中，分别有53头（包括5头具有人兽共患的十二指肠贾第虫A基因型）和17头（其中1头具有人兽共患的泛在隐孢子虫）检测到了这些原生动物。
讨论
结果表明，在巴勒莫地区，将这两种原生动物传播给农民的风险可以忽略不计，尽管由于在犊牛中鉴定出人类基因型/物种，不能认为风险为零。
Zotero 插件