Jiangxi Provincial Key Laboratory for Animal Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, Jiangxi Province 330045, China.
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, China.
Korean J Parasitol. 2020 Dec;58(6):681-687. doi: 10.3347/kjp.2020.58.6.681. Epub 2020 Dec 29.
Giardia lamblia is a common enteric pathogen associated with diarrheal diseases. There are some reports of G. lamblia infection among different breeds of cattle in recent years worldwide. However, it is yet to know whether cattle in Jiangxi province, southeastern China is infected with G. lamblia. The objectives of the present study were to investigate the prevalence and examine the multilocus genotypes of G. lamblia in cattle in Jiangxi province. A total of 556 fecal samples were collected from 3 cattle breeds (dairy cattle, beef cattle, and buffalo) in Jiangxi province, and the prevalence and genotypes of G. lamblia were determined by the nested PCR amplification of the beta-giardin (bg) gene. A total of 52 samples (9.2%) were positive for G. lamblia. The highest prevalence of G. lamblia was detected in dairy cattle (20.0%), followed by that in beef cattle (6.4%), and meat buffalo (0.9%). Multilocus sequence typing of G. lamblia was performed based on sequences of the bg, triose phosphate isomerase and glutamate dehydrogenase loci, and 22, 42, and 52 samples were amplifiable, respectively, forming 15 MLGs. Moreover, one mixed G. lamblia infection (assemblages A and E) was found in the present study. Altogether, 6 novel assemblage E subtypes (E41*-E46*) were identified for the first time. These results not only provided baseline data for the control of G. lamblia infection in cattle in this southeastern province of China, but also enriched the molecular epidemiological data and genetic diversity of G. lamblia in cattle.
蓝氏贾第鞭毛虫是一种常见的肠道病原体，与腹泻病有关。近年来，世界范围内有一些关于不同品种牛感染蓝氏贾第鞭毛虫的报道。然而，目前尚不清楚中国东南部江西省的牛是否感染了蓝氏贾第鞭毛虫。本研究的目的是调查江西省牛感染蓝氏贾第鞭毛虫的流行情况，并检测其多基因座基因型。从江西省 3 个牛品种（奶牛、肉牛和水牛）中采集了 556 份粪便样本，通过β-贾第虫（bg）基因的巢式 PCR 扩增来确定蓝氏贾第鞭毛虫的流行情况和基因型。共有 52 份（9.2%）样本为蓝氏贾第鞭毛虫阳性。蓝氏贾第鞭毛虫的最高流行率出现在奶牛中（20.0%），其次是肉牛（6.4%）和肉用水牛（0.9%）。根据 bg、磷酸丙糖异构酶和谷氨酸脱氢酶基因序列对蓝氏贾第鞭毛虫进行多基因座序列分型，共扩增了 22、42 和 52 个样本，分别形成 15 个 MLG。此外，本研究还发现了一种混合蓝氏贾第鞭毛虫感染（A 组和 E 组）。共鉴定出 6 种新的 E 组亚型（E41*-E46*）。这些结果不仅为控制中国东南部江西省牛的蓝氏贾第鞭毛虫感染提供了基线数据，还丰富了牛蓝氏贾第鞭毛虫的分子流行病学数据和遗传多样性。
