Government Office of Győr-Moson-Sopron County Policy Administration Services of Public Health, Győr, Hungary,
Parasitol Res. 2014 Jul;113(7):2551-7. doi: 10.1007/s00436-014-3905-x. Epub 2014 Apr 30.
Hungary has a large number of thermal baths and spa facilities which attract hundreds of thousands of tourists annually. Until recently, however, the free-living amoebae were not of public health concern. Genotyping of Acanthamoeba species, potential agents of keratitis and granulomatous encephalitis, was carried out in 20 Hungarian public baths for the first time to assess the incidence and molecular diversity of the genus in the country. Our results show that 6.7% of the samples were positive for Acanthamoeba. Of these positive samples, 6.5 and 7% was from sterilized and unsterilized pools, respectively. The 18S rRNA gene investigation of the nine Acanthamoeba strains found reveals that seven belong to the hazardous T4 genotype. The remaining two samples were of the T15 type. All the strains kept growing at 36 °C. Our results underline the need to develop a control system for free-living amoebae and supervise the disinfection of Hungarian public baths.
匈牙利拥有大量的温泉和水疗设施，每年吸引数十万游客。然而，直到最近，自由生活的阿米巴虫才没有引起公众的健康关注。为了评估该属在该国的发病率和分子多样性，首次对 20 个匈牙利公共浴场中的棘阿米巴属进行了潜在角膜炎和肉芽肿性脑炎病原体的基因分型。我们的研究结果表明，6.7%的样本对棘阿米巴虫呈阳性。在这些阳性样本中，分别有 6.5%和 7%来自已消毒和未消毒的泳池。对发现的 9 株棘阿米巴虫的 18S rRNA 基因研究表明，其中 7 株属于危险的 T4 基因型。其余两个样本属于 T15 型。所有菌株均在 36°C 下保持生长。我们的研究结果强调需要建立一个针对自由生活的阿米巴虫的控制系统，并监督匈牙利公共浴场的消毒。
