Karimi Azadeh, Kheirandish Farnaz, Javadi Mamaghani Amirreza, Taghipour Niloofar, Mousavi Syedeh Fatemeh, Aghajani Ali, Zebardast Nozhat, Faraji Majid, Fallahi Shirzad
Department of Medical Parasitology and Mycology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.
Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.
Parasite Epidemiol Control. 2023 May 25;22:e00308. doi: 10.1016/j.parepi.2023.e00308. eCollection 2023 Aug.
spp. is opportunistic amoeba that resides in water, soil, and air. Some pathogenic genotypes of the genus of can cause granulomatous amoebic encephalitis (GAE) in people with a defective immune system. The parasite can also cause keratitis (AK) among contact lens users. This study was conducted to isolate and identify the genotypes in water resources in Lorestan province, western Iran.
Collected 72 water samples from surface and groundwater (springs and aqueducts) in Lorestan province. Samples were filtered and cultured in non-nutrient 1.5% agar medium covered with at 25 °C. DNA extraction was done and the PCR reaction was performed to detect the spp. The positive PCR products were sequenced to determine the genotypes of
Out of 72 examined water samples, 23.61% were positive for sp. by PCR. From PCR-positive samples, 8 (47.05%) samples were T4 genotypes and others were other genotypes (T1-T23). Therefore, approximately half of the genotypes belong to the pathogenic T4 genotype.
The water examined samples in western provinces of Iran have the potential risk factor for public health. Therefore, the efforts of healthcare providers are needed to identify, train, and prevention from human infections.
棘阿米巴属是一种机会性变形虫，存在于水、土壤和空气中。该属的一些致病基因型可在免疫系统有缺陷的人群中引起肉芽肿性阿米巴脑炎（GAE）。这种寄生虫还可在隐形眼镜使用者中引起棘阿米巴角膜炎（AK）。本研究旨在分离和鉴定伊朗西部洛雷斯坦省水资源中的棘阿米巴基因型。
从洛雷斯坦省的地表水和地下水（泉水和渡槽）中采集72份水样。将样品过滤并在覆盖有大肠杆菌的1.5%非营养琼脂培养基中于25℃培养。进行DNA提取并进行PCR反应以检测棘阿米巴属。对PCR阳性产物进行测序以确定棘阿米巴的基因型。
在72份检测的水样中，23.61%的水样通过PCR检测棘阿米巴属呈阳性。在PCR阳性样本中，8份（47.05%）样本为T4基因型，其他为其他棘阿米巴基因型（T1 - T23）。因此，大约一半的基因型属于致病性T4基因型。
伊朗西部省份检测的水样存在公共卫生潜在风险因素。因此，需要医疗保健提供者努力识别、培训并预防人类感染。
