Levdik T I
Arkh Patol. 1989;51(7):81-2.
The method of the paraffin or celloidin-paraffin sections and histoautoradiogram accelerated staining with Harris haematoxylin is suggested. The quality of staining with this haematoxylin of all organs and tissues of rats sacrificed under the ether narcosis or after natural death is evaluated. All the histoautoradiogram treatment (exposition, development, fixation, washing and staining) is being carried out in the same cassette this excluding the possibility of the photolayer and section damage. Harris haematoxylin enables the elective nuclear staining and this allows, without detriment to the quality, using the cassette method of staining with this reagent in laboratories with a large bulk of work and recommending it for elaboration of the technical standards.
建议采用石蜡或火棉胶 - 石蜡切片法以及用苏木精快速染色的组织放射自显影片。对在乙醚麻醉下处死或自然死亡的大鼠的所有器官和组织用这种苏木精进行染色质量评估。所有组织放射自显影片处理（曝光、显影、固定、冲洗和染色）都在同一个暗盒中进行，这样可排除光层和切片受损的可能性。苏木精能实现选择性细胞核染色，这使得在工作量大的实验室中，在不影响质量的情况下，可以使用该试剂的暗盒染色法，并推荐将其用于制定技术标准。
