Automated hematoxylin and eosin staining for large volumes of tissue.

In order to facilitate the large volume of hematoxylin and eosin (H & E) slides generated by 18 experiments from various programs at the National Center for Toxicological Research, a rapid staining method became imperative. Automated staining with the Gam Rad Stainomatic was decided upon using Gill's no. 1 hematoxylin with a 30-minute staining schedule. Presently, 1,080 slides per instrument per day are stained with a good dependable stain. The cost of this H & E staining method is 0.15 cents per slide.