Silva Mariana Sá E, Weiss Marcelo, Brum Mário Celso Sperotto, Dos Anjos Bruno Leite, Torres Fabricio Dias, Weiblen Rudi, Flores Eduardo Furtado
Departamento de Medicina Veterinária Preventiva (DMVP), Universidade Federal de Santa Maria (UFSM), Santa Maria 97105-900, RS, Brazil.
J Vet Diagn Invest. 2010 Jul;22(4):603-6. doi: 10.1177/104063871002200417.
Bovine papillomaviruses (BPVs) are widespread pathogens mainly associated with benign, self-limiting, cutaneous lesions (warts). At least 8 viral types, defined by serology or nucleotide sequences of the L1 gene, have been identified to date. Different serotypes are associated with the specific type and morphology of the lesion and with particular geographical regions. This article describes the molecular identification of papillomaviruses from Brazilian cattle (n = 48) and horses (n = 1) through partial amplification and sequencing of the L1 gene. Bovine papillomavirus-1 (BPV-1) was identified in warts from 29 cattle (59%), BPV-6 from 9 cattle (18%), and BPV-2 in 8 lesions (16%). Warts of 2 cattle harbored L1 sequences of a new BPV type (BAA5), otherwise identified almost exclusively in healthy skin. The newly proposed BPV type "BR-UEL-4" was identified in a sarcoid tumor of a horse. Thus, the present report provides information on the main types of BPV involved in bovine papillomatosis in Brazil and reveals a new viral type associated with equine sarcoid, which to date has been attributed exclusively to BPV-1 and BPV-2.
牛乳头瘤病毒（BPVs）是广泛传播的病原体，主要与良性、自限性的皮肤病变（疣）相关。迄今为止，已通过血清学或L1基因的核苷酸序列鉴定出至少8种病毒类型。不同的血清型与病变的特定类型和形态以及特定地理区域相关。本文描述了通过对L1基因进行部分扩增和测序，对来自巴西牛（n = 48）和马（n = 1）的乳头瘤病毒进行分子鉴定的过程。在29头牛（59%）的疣中鉴定出牛乳头瘤病毒1型（BPV-1），9头牛（18%）的疣中鉴定出BPV-6，8个病变（16%）中鉴定出BPV-2。2头牛的疣中含有一种新的BPV类型（BAA5）的L1序列，否则几乎只在健康皮肤中鉴定到。在一匹马的肉瘤样肿瘤中鉴定出了新提出的BPV类型“BR-UEL-4”。因此，本报告提供了关于巴西牛乳头瘤病中涉及的主要BPV类型的信息，并揭示了一种与马属肉瘤相关的新病毒类型，迄今为止该病仅归因于BPV-1和BPV-2。
