Crespo Sarah Elizabeth Izzo, Lunardi Michele, Otonel Rodrigo Alejandro Arellano, Headley Selwyn Arlington, Alfieri Alice Fernandes, Alfieri Amauri Alcindo
Department of Veterinary Preventive Medicine, Laboratory of Animal Virology, Universidade Estadual de Londrina, Celso Garcia Cid Road, PR 445 km 380, Londrina, Paraná, 86051-990, Brazil.
Laboratory of Veterinary Microbiology, Universidade de Cuiabá, 3100 Ave Beira Rio, Cuiabá, Mato Grosso, 78065-900, Brazil.
Virus Genes. 2019 Oct;55(5):682-687. doi: 10.1007/s11262-019-01694-8. Epub 2019 Aug 3.
Currently, bovine papillomavirus types are divided into five genera, namely, Deltapapillomavirus, Epsilonpapillomavirus, Xipapillomavirus, Dyoxipapillomavirus, and Dyokappapapillomavirus. In the recent decades, the characterization of numerous putative and novel bovine papillomavirus types from cattle in several geographic regions, has revealed the occurrence of a high viral diversity. In this study, we describe the identification and characterization of a putative new bovine papillomavirus type within species Xipapillomavirus 1 of Xipapillomavirus genus. The detection of the viral types identified in the skin warts was obtained by polymerase chain reaction assays targeting the L1 gene, followed by direct sequencing of the generated amplicons. The partial L1 sequences revealed that bovine papillomavirus types 6, 10, and 11, the putative new bovine papillomavirus type designated BPV/CHI-SW2, and an unreported putative new bovine papillomavirus type (named BPV/BR-UEL08) were associated with cutaneous papillomatosis in the cows from the dairy herd investigated. Phylogenetic reconstruction based on the L1 gene revealed that the BPV/BR-UEL08 isolate clustered with other bovine papillomaviruses classified in the Xipapillomavirus genus, being closely related to representatives of the species Xipapillomavirus 1. Investigations focusing on the molecular epidemiology of bovine papillomaviruses related to clinical outcomes in cattle are of fundamental importance to determine the actual genetic diversity and prevalent viral types to be included in vaccines for cattle.
目前，牛乳头瘤病毒类型分为五个属，即δ乳头瘤病毒属、ε乳头瘤病毒属、剑乳头瘤病毒属、双氧剑乳头瘤病毒属和双氧κ乳头瘤病毒属。在最近几十年里，对来自几个地理区域牛群中众多假定的和新型牛乳头瘤病毒类型的特征分析，揭示了高度的病毒多样性。在本研究中，我们描述了剑乳头瘤病毒属剑乳头瘤病毒1种内一种假定的新型牛乳头瘤病毒类型的鉴定和特征。通过针对L1基因的聚合酶链反应分析，随后对产生的扩增子进行直接测序，检测在皮肤疣中鉴定出的病毒类型。部分L1序列显示，牛乳头瘤病毒6型、10型和11型、指定为BPV/CHI-SW2的假定新型牛乳头瘤病毒类型以及一种未报道的假定新型牛乳头瘤病毒类型（命名为BPV/BR-UEL08）与所调查奶牛群中奶牛的皮肤乳头瘤病有关。基于L1基因的系统发育重建表明，BPV/BR-UEL08分离株与剑乳头瘤病毒属中分类的其他牛乳头瘤病毒聚集在一起，与剑乳头瘤病毒1种的代表密切相关。关注与牛临床结果相关的牛乳头瘤病毒分子流行病学的研究对于确定实际的遗传多样性和牛疫苗中应包含的流行病毒类型至关重要。
