Department of Research Development, Institute of Jinan Red Ginseng, Jinan, Republic of Korea.
Department of Oriental Medicine Resources, College of Environmental and Bioresource Sciences, Chonbuk National University, Republic of Korea.
Evid Based Complement Alternat Med. 2014;2014:450572. doi: 10.1155/2014/450572. Epub 2014 Mar 24.
Cryptotanshinone (CT), a major tanshinone of medicinal plant Salvia miltiorrhiza Bunge, demonstrated strong antibacterial activity against clinic isolated methicillin and vancomycin-resistant Staphylococcus aureus (MRSA and VRSA) in this experiment. The CT was determined against clinic isolated MRSA 1-16 with MIC and MBC values ranging from 4 to 32 and 8 to 128 μ g/mL; for MSSA 1-2 from 16 to 32 μ g/mL and 64 to 128 μ g/mL; for VRSA 1-2 from 2 to 4 μ g/mL and 4 to 16 μ g/mL, respectively. The range of MIC50 and MIC90 of CT was 0.5-8 μ g/mL and 4-64 μ g/mL, respectively. The combination effects of CT with antibiotics were synergistic (FIC index <0.5) against most of tested clinic isolated MRSA, MSSA, and VRSA except additive, MRSA 4 and 16 in oxacillin, MRSA 6, 12, and 15 in ampicillin, and MRSA 6, 11, and 15 in vancomycin (FIC index < 0.75-1.0). Furthermore, a time-kill study showed that the growth of the tested bacteria was completely attenuated after 2-6 h of treatment with the 1/2 MIC of CT, regardless of whether it was administered alone or with ampicillin, oxacillin, or vancomycin. The results suggest that CT could be employed as a natural antibacterial agent against multidrug-resistant pathogens infection.
隐丹参酮（CT）是药用植物丹参中的一种主要的丹参酮，在本实验中对临床分离的耐甲氧西林金黄色葡萄球菌（MRSA 和 VRSA）表现出很强的抗菌活性。CT 对临床分离的 MRSA 1-16 的 MIC 和 MBC 值范围分别为 4 至 32 和 8 至 128μ g/mL；对 MSSA 1-2 的 MIC 值范围为 16 至 32μ g/mL，MBC 值范围为 64 至 128μ g/mL；对 VRSA 1-2 的 MIC 值范围为 2 至 4μ g/mL，MBC 值范围为 4 至 16μ g/mL。CT 的 MIC50 和 MIC90 范围分别为 0.5-8μ g/mL 和 4-64μ g/mL。除了相加作用（FIC 指数<0.5）外，CT 与抗生素联合使用对大多数测试的临床分离的 MRSA、MSSA 和 VRSA 具有协同作用（FIC 指数<0.5），除了对耐苯唑西林的 MRSA 4 和 16、耐氨苄西林的 MRSA 6、12 和 15、耐万古霉素的 MRSA 6、11 和 15 为相加作用（FIC 指数<0.75-1.0）。此外，时间杀伤研究表明，在用 CT 的 1/2 MIC 治疗 2-6 小时后，无论是否单独使用或与氨苄西林、苯唑西林或万古霉素联合使用，测试细菌的生长均完全减弱。这些结果表明，CT 可以作为一种天然的抗菌剂用于治疗多重耐药病原体感染。
