Sreepian Preeyaporn M, Rattanasinganchan Panthip, Sreepian Apichai
Faculty of Medical Technology, Rangsit University, Pathum Thani 12000, Thailand.
Faculty of Medical Technology, Huachiew Chalermprakiet University, Samut Prakan 10540, Thailand.
Saudi Pharm J. 2023 Jun;31(6):1094-1103. doi: 10.1016/j.jsps.2023.03.020. Epub 2023 Apr 3.
The increasing incidence of methicillin-resistant is a major public health concern. Recently, the performance of essential oil (CHEO) has been shown to contain broad-spectrum antibacterial activity. Therefore, this study aims to determine the antibacterial activity of CHEO alone and in combination with gentamicin against panels of clinical isolates of methicillin-susceptible (MSSA, = 45) and methicillin-resistant (MRSA, = 40). Antibiotic susceptibility testing revealed multidrug-resistant (MDR) patterns among 3 MSSA isolates and 39 MRSA isolates, indicating that the clinical MRSA isolates were associated with MDR ( < 0.05). For the drug resistant isolates, resistance was observed toward most antibiotics, except for chloramphenicol, trimethoprim-sulfamethoxazole, linezolid, and vancomycin. Antibacterial screening by disk diffusion demonstrated that CHEO alone had certain antibacterial activity toward all MSSA isolates (IZD: 16.0 ± 4.7 mm) and MRSA isolates (IZD: 16.5 ± 4.2 mm) ( > 0.05). The MIC values of CHEO are 18.3 ± 6.1 mg/mL in MSSA isolates and 17.9 ± 6.9 mg/mL in MRSA isolates ( > 0.05). The antibacterial activity of CHEO demonstrated the bactericidal effect with MIC index 1.0-1.4. Time-killing kinetics revealed that CHEO at 1 × MIC completely killed MSSA and MRSA within 12 h. Moreover, the checkerboard titration demonstrated the synergistic and additive interactions of CHEO with gentamicin with FIC index 0.012-0.625. CHEO against human epidermal keratinocyte; HaCaT cell line demonstrated the IC value at 2.15 mg/mL. The use of CHEO as an alternative antibacterial agent would reduce the emergence of resistant bacteria, especially MDR MRSA.
耐甲氧西林金黄色葡萄球菌发病率的不断上升是一个重大的公共卫生问题。最近，肉桂叶精油（CHEO）已被证明具有广谱抗菌活性。因此，本研究旨在确定CHEO单独以及与庆大霉素联合对甲氧西林敏感金黄色葡萄球菌（MSSA，n = 45）和耐甲氧西林金黄色葡萄球菌（MRSA，n = 40）临床分离株的抗菌活性。抗生素敏感性测试显示，3株MSSA分离株和39株MRSA分离株呈现多重耐药（MDR）模式，表明临床MRSA分离株与MDR相关（P < 0.05）。对于耐药分离株，除氯霉素、甲氧苄啶 - 磺胺甲恶唑、利奈唑胺和万古霉素外，对大多数抗生素均有耐药性。纸片扩散法抗菌筛选表明，CHEO单独对所有MSSA分离株（抑菌圈直径：16.0 ± 4.7 mm）和MRSA分离株（抑菌圈直径：16.5 ± 4.2 mm）具有一定的抗菌活性（P > 0.05）。CHEO在MSSA分离株中的最低抑菌浓度（MIC）值为18.3 ± 6.1 mg/mL，在MRSA分离株中为17.9 ± 6.9 mg/mL（P > 0.05）。CHEO的抗菌活性表现为杀菌作用，MIC指数为1.0 - 1.4。时间杀菌动力学表明，1×MIC的CHEO在12小时内可完全杀灭MSSA和MRSA。此外，棋盘滴定法表明CHEO与庆大霉素具有协同和相加相互作用，FIC指数为0.012 - 0.625。CHEO对人表皮角质形成细胞；HaCaT细胞系的IC值为2.15 mg/mL。使用CHEO作为替代抗菌剂将减少耐药菌的出现，尤其是多重耐药的MRSA。
