Safari Majid, Abtahi Hamid, Chehreii Shima, Fahimirad Shohreh
Department of Microbiology, Faculty of Basic Sciences, Islamic Azad University, Arak, Iran.
Molecular and Medicine Research Center, Arak University of Medical Sciences, Arak, Iran.
Protein Pept Lett. 2025;32(1):34-43. doi: 10.2174/0109298665327474241112093601.
Methicillin-resistant (MRSA) is a significant and prevalent pathogen that poses a major challenge in healthcare environments. In light of the growing threat posed by multidrug-resistant organisms like MRSA, there is an urgent need for alternative therapeutic strategies. One promising avenue of research involves the use of antimicrobial peptides (AMPs). These naturally occurring molecules, which are part of the innate immune response in many organisms, have garnered attention for their ability to combat a wide range of pathogens.
This study aimed to produce recombinant versions of Ib-AMP and Oncorhyncin II and to evaluate their combined effects against MRSA (NCTC10442).
served as the expression host for the synthesized variants of the Ib-AMP and Oncorhyncin II genes. The antimicrobial efficacy of these peptides against MRSA (NCTC1042) was evaluated using a comprehensive methodology that encompassed the determination of the minimum inhibitory concentration (MIC), the performance of time-kill assays, and the analysis of growth kinetics.
The individual antimicrobial activities of Ib-AMP and Oncorhyncin II were assessed, revealing minimum inhibitory concentrations (MICs) of 27.75 μg/mL and 40.125 μg/mL against S. aureus (MRSA) (NCTC10442), respectively. The application of a checkerboard assay to evaluate the combination of these antimicrobial peptides (AMPs) demonstrated a synergistic interaction, which was further validated through time-kill and growth kinetic studies. When administered at double the MIC, a significant reduction in the log CFU/mL of MRSA (NCTC 10442) was observed, underscoring the synergistic bacteriostatic effect mediated by the fractional inhibitory concentration (FIC) index of the two peptides.
Antimicrobial peptides (AMPs) have attracted significant interest owing to the growing intricacy of microbial infections. They constitute a promising category of novel antibiotics that warrant further investigation for the treatment of infections and the enhancement of wound healing. Although certain AMPs can operate autonomously, others may necessitate a synergistic approach alongside conventional antibiotics. Studies examining the combined efficacy of Oncorhyncin II and Ib-AMP against MRSA have revealed their effectiveness.
耐甲氧西林金黄色葡萄球菌（MRSA）是一种重要且普遍存在的病原体，在医疗环境中构成重大挑战。鉴于MRSA等多重耐药生物体带来的威胁日益增加，迫切需要替代治疗策略。一个有前景的研究途径涉及使用抗菌肽（AMPs）。这些天然存在的分子是许多生物体固有免疫反应的一部分，因其对抗多种病原体的能力而受到关注。
本研究旨在生产重组版的Ib-AMP和大麻哈鱼抗菌肽II（Oncorhyncin II），并评估它们对MRSA（NCTC10442）的联合作用。
作为Ib-AMP和大麻哈鱼抗菌肽II基因合成变体的表达宿主。使用包括测定最低抑菌浓度（MIC）、进行时间杀菌试验和分析生长动力学的综合方法，评估这些肽对MRSA（NCTC1042）的抗菌效果。
评估了Ib-AMP和大麻哈鱼抗菌肽II的个体抗菌活性，结果显示它们对金黄色葡萄球菌（MRSA）（NCTC10442）的最低抑菌浓度（MIC）分别为27.75μg/mL和40.125μg/mL。应用棋盘法评估这些抗菌肽（AMPs）的组合显示出协同相互作用，通过时间杀菌和生长动力学研究进一步得到验证。当以两倍MIC给药时，观察到MRSA（NCTC 10442）的log CFU/mL显著降低，突出了两种肽的分数抑菌浓度（FIC）指数介导的协同抑菌作用。
由于微生物感染的复杂性不断增加，抗菌肽（AMPs）引起了极大关注。它们构成了一类有前景的新型抗生素，值得进一步研究用于治疗感染和促进伤口愈合。尽管某些抗菌肽可以自主发挥作用，但其他抗菌肽可能需要与传统抗生素采用协同方法。研究检查大麻哈鱼抗菌肽II和Ib-AMP对MRSA的联合疗效已显示出它们的有效性。
