Ranadive N S, Menon I A, Shirwadkar S, Persad S D
Department of Pathology, University of Toronto, Ontario, Canada.
Inflammation. 1989 Oct;13(5):483-94. doi: 10.1007/BF00916756.
The present studies were undertaken to quantitate the initial inflammatory response produced by the photo-generated reactive species in rabbit skin. Rose bengal (RB), a photosensitizer dye, was injected into the skin sites at various concentrations and exposed to UV-visible light for 30-120 min. The increase in vascular permeability and the accumulation of PMNs were investigated using 125I-labeled albumin and 51Cr-labeled PMNs. RB at a concentration of 1 nmol with 120-min exposure to light enhanced vascular permeability by 3.7 times and accumulation of PMNs by 3.3 times. As low as 0.01 nmol of RB produced discernible effects. beta-Carotene (0.1 nmole) inhibited the inflammatory response by 75-100%, suggesting that the reactive species involved in this response was predominantly singlet oxygen. The increase in vascular permeability was inhibited by 48-70% by 25 micrograms of chlorpheniramine maleate. It is therefore suggested that histamine plays a major role in the initial vascular response. The studies demonstrate that this rabbit model is suitable for the quantitation of photoinduced inflammatory response which is not observable by gross anatomic procedures.
本研究旨在定量分析光生成反应性物种在兔皮肤中引发的初始炎症反应。孟加拉玫瑰红（RB），一种光敏染料，以不同浓度注射到皮肤部位，并暴露于紫外-可见光下30 - 120分钟。使用125I标记的白蛋白和51Cr标记的中性粒细胞来研究血管通透性的增加和中性粒细胞的聚集。浓度为1 nmol的RB在光照120分钟后，血管通透性提高了3.7倍，中性粒细胞聚集增加了3.3倍。低至0.01 nmol的RB也产生了明显的效果。β-胡萝卜素（0.1 nmol）可将炎症反应抑制75 - 100%，这表明参与该反应的反应性物种主要是单线态氧。25微克马来酸氯苯那敏可将血管通透性的增加抑制48 - 70%。因此，提示组胺在初始血管反应中起主要作用。这些研究表明，该兔模型适用于定量分析光诱导的炎症反应，而这是通过大体解剖程序无法观察到的。
