Iqbal S Z, Asi M R, Ariño A
a Department of Applied Chemistry , GC University Faisalabad , 38000, Faisalabad , Pakistan.
Food Addit Contam Part B Surveill. 2011;4(4):282-8. doi: 10.1080/19393210.2011.637237.
A total of 178 milk samples (94 of buffalo and 84 of cow) were randomly taken from Punjab and the North West Frontier Province (NWFP) of Pakistan (n = 89 in each province) and analysed for the presence of aflatoxin M1 (AFM1) by HPLC-FLD. From Punjab about 46% of buffalo's and 49% of cow's milks were contaminated with AFM1 as compared with 52% and 51% for milk samples from NWFP, respectively. Overall, the mean AFM1 concentration was 0.046 µg kg(-1) with a maximum of 0.350 µg kg(-1). All samples complied with the Codex Alimentarius limit of 0.50 µg kg(-1) for AFM1 in milk, but 16.3% of samples exceeded the European Union maximum level of 0.05 µg kg(-1). Another set of 415 buffalo's and cow's milk samples (213 morning milks and 202 evening milks) were analysed. Statistical analysis revealed significant differences (p < 0.05) between mean AFM1 concentrations in milk during the morning (0.043 µg kg(-1)) and the evening (0.028 µg kg(-1)) lactation times.
从巴基斯坦旁遮普省和西北边境省（NWFP）随机采集了总共178份牛奶样本（94份水牛奶和84份牛奶）（每个省89份），并通过高效液相色谱 - 荧光检测法（HPLC - FLD）分析黄曲霉毒素M1（AFM1）的存在情况。旁遮普省约46%的水牛奶和49%的牛奶被AFM1污染，而NWFP省牛奶样本的这一比例分别为52%和51%。总体而言，AFM1的平均浓度为0.046微克/千克，最高为0.350微克/千克。所有样本均符合食品法典委员会规定的牛奶中AFM1含量0.50微克/千克的限量，但16.3%的样本超过了欧盟0.05微克/千克的最高限量。另外分析了415份水牛奶和牛奶样本（213份晨奶和202份晚奶）。统计分析显示，早晨（0.043微克/千克）和晚上（0.028微克/千克）泌乳期牛奶中AFM1的平均浓度存在显著差异（p < 0.05）。
