Sánchez-Ortiga Emilio, Doblas Ana, Saavedra Genaro, Martínez-Corral Manuel, Garcia-Sucerquia Jorge
Appl Opt. 2014 Apr 1;53(10):2058-66. doi: 10.1364/AO.53.002058.
The utilization of microscope objectives (MOs) in digital holographic microscopy (DHM) has associated effects that are not present in conventional optical microscopy. The remaining phase curvature, which can ruin the quantitative phase imaging, is the most evident and analyzed. As phase imaging is considered, this interest has made possible the development of different methods of overcoming its undesired consequences. Additionally to the effects in phase imaging, there exist a set of less obvious conditions that have to be accounted for as MOs are utilized in DHM to achieve diffraction-limit operation. These conditions have to be considered even in the case in which only amplitude or intensity imaging is of interest. In this paper, a thorough analysis of the physical parameters that control the appropriate utilization of MOs in DHM is presented. A regular DHM system is theoretically modeled on the basis of the imaging theory. The Fourier spectrum of the recorded hologram is analyzed to evaluate the performance of the DHM. A set of the criteria that consider the microscope features and the recording parameters to achieve DHM operation at the diffraction limit is derived. Numerical modeling and experimental results are shown to validate our findings.
在数字全息显微镜（DHM）中使用显微镜物镜（MO）会产生一些传统光学显微镜中不存在的相关效应。剩余相位曲率会破坏定量相位成像，这是最明显且已被分析的效应。随着相位成像受到关注，这种关注使得开发不同方法来克服其不良后果成为可能。除了对相位成像的影响外，在DHM中使用MO以实现衍射极限操作时，还存在一组不太明显的条件需要考虑。即使只对幅度或强度成像感兴趣，这些条件也必须予以考虑。本文对控制DHM中MO合理使用的物理参数进行了深入分析。基于成像理论对常规DHM系统进行了理论建模。分析记录全息图的傅里叶频谱以评估DHM的性能。推导了一组考虑显微镜特性和记录参数以在衍射极限下实现DHM操作的标准。给出了数值建模和实验结果以验证我们的发现。
