Trujillo Carlos, Castañeda Raúl, Piedrahita-Quintero Pablo, Garcia-Sucerquia Jorge
Appl Opt. 2016 Dec 20;55(36):10299-10306. doi: 10.1364/AO.55.010299.
An automatic method that fully compensates the quantitative phase measurements in off-axis digital holographic microscopy (DHM) is presented. The two main perturbations of the quantitative phase measurements in off-axis DHM are automatically removed. While the curvature phase flaw introduced by the microscope objective is avoided by the use of an optimized telecentric imaging system for the recording of the holograms, the remaining phase perturbation due to the tilt of the reference wave is removed by the automatic computation of a digital compensating reference wave. The method has been tested on both nonbiological and biological samples with and improving on the quality of the recovered phase maps.
提出了一种在离轴数字全息显微镜（DHM）中完全补偿定量相位测量的自动方法。离轴DHM中定量相位测量的两个主要干扰因素被自动消除。通过使用优化的远心成像系统来记录全息图，避免了显微镜物镜引入的曲率相位缺陷，而由于参考波倾斜引起的剩余相位干扰则通过自动计算数字补偿参考波来消除。该方法已在非生物和生物样品上进行了测试，恢复的相位图质量得到了改善。
