Do Thanh-Tri, Quach-Van Toan-Em, Nguyen Thanh-Cong, Show Pau Loke, Nguyen Tran Minh-Ly, Huynh Duc-Hoan, Tran Dai-Long, Melkonian Michael, Tran Hoang-Dung
Faculty of Biology-Biotechnology, University of Science, Vietnam National University Ho Chi Minh City, 227 Nguyen Van Cu Street, District 5, Ho Chi Minh City 72711, Vietnam.
Faculty of Biology, Ho Chi Minh City University of Education, 280 An Duong Vuong Street, District 5, Ho Chi Minh City 72711, Vietnam.
Bioengineering (Basel). 2023 May 16;10(5):596. doi: 10.3390/bioengineering10050596.
Light-emitting diodes are increasingly used as artificial light sources in cultivation due to the fact of their energy advantages. The immobilized cultivation of in pilot-scale angled twin-layer porous substrate photobioreactors (TL-PSBRs) was initially performed with a 14/10 h light/dark cycle and showed relatively low biomass growth and astaxanthin accumulation. In this study, the illumination time with red and blue LEDs at a light intensity of 120 µmol photons m s was increased to 16-24 h per day. With a light/dark cycle of 22/2 h, the biomass productivity of the algae was 7.5 g m day, 2.4 times higher than in the 14/10 h cycle. The percentage of astaxanthin in the dry biomass was 2%, and the total amount of astaxanthin was 1.7 g m. Along with the increase in light duration, adding 10 or 20 mM NaHCO to the BG11-H culture medium over ten days of cultivation in angled TL-PSBRs did not increase the total amount of astaxanthin compared with only CO addition at a flow rate of 3.6 mg min to the culture medium. Adding NaHCO with a 30-80 mM concentration inhibited algal growth and astaxanthin accumulation. However, adding 10-40 mM NaHCO caused algal cells to accumulate astaxanthin at a high percentage in dry weight after the first four days in TL-PSBRs.
由于具有能源优势，发光二极管越来越多地被用作栽培中的人工光源。在中试规模的倾斜双层多孔基质光生物反应器（TL-PSBRs）中对[具体藻类名称未给出]进行固定化培养，最初采用14/10小时的光/暗循环，结果显示生物量增长和虾青素积累相对较低。在本研究中，将红色和蓝色发光二极管在光强为120 μmol光子·m⁻²·s⁻¹时的光照时间增加到每天16 - 24小时。在22/2小时的光/暗循环下，藻类的生物量生产力为7.5 g·m⁻²·天，比14/10小时循环时高2.4倍。干生物量中虾青素的百分比为2%，虾青素总量为1.7 g·m⁻²。随着光照时间的增加，在倾斜的TL-PSBRs中培养十天期间，向BG11-H培养基中添加10或20 mM NaHCO，与仅以3.6 mg·min⁻¹的流速向培养基中添加CO₂相比，虾青素总量并未增加。添加浓度为30 - 80 mM的NaHCO会抑制藻类生长和虾青素积累。然而，添加10 - 40 mM NaHCO会使藻类细胞在TL-PSBRs中培养的前四天后，干重中虾青素的积累百分比很高。
