Davis K M, Gibson L E, Haselton F R, Wright D W
Department of Chemistry, Vanderbilt University, Station B 351822, Nashville, TN 37235-1822, USA.
Analyst. 2014 Jun 21;139(12):3026-31. doi: 10.1039/c4an00338a. Epub 2014 May 1.
Lateral flow immunochromatographic rapid diagnostic tests (RDTs) are the primary form of medical diagnostic used for malaria in underdeveloped nations. Unfortunately, many of these tests do not detect asymptomatic malaria carriers. In order for eradication of the disease to be achieved, this problem must be solved. In this study, we demonstrate enhancement in the performance of six RDT brands when a simple sample-processing step is added to the front of the diagnostic process. Greater than a 4-fold RDT signal enhancement was observed as a result of the sample processing step. This lowered the limit of detection for RDT brands to submicroscopic parasitemias. For the best performing RDTs the limits of detection were found to be as low as 3 parasites per μL. Finally, through individual donor samples, the correlations between donor source, WHO panel detection scores and RDT signal intensities were explored.
侧向流动免疫层析快速诊断测试（RDTs）是欠发达国家用于疟疾诊断的主要医学诊断形式。不幸的是，许多此类测试无法检测出无症状的疟疾携带者。为了实现该疾病的根除，必须解决这个问题。在本研究中，我们证明，当在诊断过程前端添加一个简单的样本处理步骤时，六个RDT品牌的性能得到了提升。由于样本处理步骤，观察到RDT信号增强了4倍以上。这将RDT品牌的检测限降低到亚显微寄生虫血症水平。对于性能最佳的RDTs，发现检测限低至每微升3个寄生虫。最后，通过个体捐赠者样本，探索了捐赠者来源、世卫组织专家组检测分数与RDT信号强度之间的相关性。
