Zhang Xiaopo, Tan Yifeng, Li Youbin, Jin Lifeng, Wei Na, Wu Haifeng, Ma Guoxu, Zheng Qingxia, Tian Yu, Yang Junshan, Zhang Junqing, Xu Xudong
School of Pharmaceutical Science, Hainan Medical University.
Chem Pharm Bull (Tokyo). 2014;62(5):494-8. doi: 10.1248/cpb.c14-00056.
Six new acyclic diterpenoids named Aphanamixins A-F (1-6), together with two known compounds of nemoralisin and nemoralisin C, were isolated from the stem bark of Aphanamixis polystachya (WALL) J. N. BARKER. Their structures were established through a comprehensive analysis of NMR spectroscopic data and high resolution mass spectrometric data. The absolute configurations of carbon stereocenters were determined by means of auxiliary chiral α-methoxy-α-(trifluoromethyl)phenylacetic acid (MTPA) derivatives and circular dichroism (CD), respectively. All the new isolates were tested for their antiproliferative activity against HepG2, AGS, MCF-7, and A-549 cancer cell lines and they exhibited weak cytotoxicities (IC50>10 µM). Moreover, we highlighted that the six new diterpenoids characterized by acyclic skeleton was rarely seen in nature.
从多穗阿芳樟（Aphanamixis polystachya (WALL) J. N. BARKER）的茎皮中分离出六种新的无环二萜类化合物，命名为阿芳樟素A - F（1 - 6），以及两种已知化合物nemoralisin和nemoralisin C。通过对核磁共振光谱数据和高分辨率质谱数据的综合分析确定了它们的结构。碳立体中心的绝对构型分别通过辅助手性α - 甲氧基 - α -（三氟甲基）苯乙酸（MTPA）衍生物和圆二色性（CD）测定。测试了所有新分离物对HepG2、AGS、MCF - 7和A - 549癌细胞系的抗增殖活性，它们表现出较弱的细胞毒性（IC50>10 μM）。此外，我们强调了以无环骨架为特征的六种新二萜类化合物在自然界中很少见。
