School of Traditional Chinese Materia Medica, Guangdong Pharmaceutical University, Guangzhou 510006, China.
China Academy of Chinese·Medical Sciences, Beijing 100700, China.
Molecules. 2024 Jun 8;29(12):2733. doi: 10.3390/molecules29122733.
Four new diterpenoids, isodosins A-D (-), together with nine known compounds (-) were isolated and identified from the aerial parts of (Maxim.) Hara. The structures of the new diterpenoids were elucidated based on the analysis of HR-ESI-MS data, 1D/2D-NMR-spectroscopic data, and electronic circular dichroism (ECD) calculations. Cytotoxicities of compounds , , , , and against the HepG2 and H1975 cell lines were evaluated with the MTT assay. As a result, compounds , , and revealed higher levels of cytotoxicity against HepG2 cells than against H1975 cells. Moreover, compund demonstrated the most efficacy in inhibiting the proliferation of HepG2 cells, with an IC value of 41.13 ± 3.49 μM. This effect was achieved by inducing apoptosis in a dose-dependent manner. Furthermore, the relationships between the structures and activities of these compounds are briefly discussed.
从 （Maxim.）Hara 的地上部分分离并鉴定出四个新的二萜类化合物，异丁香素 A-D (-)，以及九个已知化合物 (-)。根据 HR-ESI-MS 数据、1D/2D-NMR 谱数据和电子圆二色性（ECD）计算，解析了新二萜的结构。用 MTT 法测定了化合物 、 、 、 、 对 HepG2 和 H1975 细胞系的细胞毒性。结果表明，化合物 、 、 和 对 HepG2 细胞的细胞毒性高于对 H1975 细胞的毒性。此外，化合物 对 HepG2 细胞的增殖抑制作用最为显著，IC 值为 41.13±3.49μM。这种作用是通过剂量依赖性诱导细胞凋亡来实现的。此外，还简要讨论了这些化合物的结构与活性之间的关系。
