Okazaki Kazuyuki, Iino Takao, Kuroda Yosuke, Taguchi Kazunori, Takahashi Hiroyuki, Ohwada Takuji, Tsurumaru Hiroto, Okubo Takashi, Minamisawa Kiwamu, Ikeda Seishi
Hokkaido Agricultural Research Center, National Agriculture and Food Research Organization.
Microbes Environ. 2014;29(2):220-3. doi: 10.1264/jsme2.me13182. Epub 2014 Apr 30.
The partial sequences of the 16S rRNA genes of 531 bacteria isolated from the main root of the sugar beet (Beta vulgaris L.) were determined and subsequently grouped into 155 operational taxonomic units by clustering analysis (≥99% identity). The most abundant phylum was Proteobacteria (72.5-77.2%), followed by Actinobacteria (9.8-16.6%) and Bacteroidetes (4.3-15.4%). Alphaproteobacteria (46.7-64.8%) was the most dominant class within Proteobacteria. Four strains belonging to Verrucomicrobia were also isolated. Phylogenetic analysis revealed that the Verrucomicrobia bacterial strains were closely related to Haloferula or Verrucomicrobium.
测定了从甜菜(Beta vulgaris L.)主根分离出的531株细菌的16S rRNA基因部分序列,随后通过聚类分析(≥99%同一性)将其归为155个操作分类单元。最丰富的门是变形菌门(72.5 - 77.2%),其次是放线菌门(9.8 - 16.6%)和拟杆菌门(4.3 - 15.4%)。α-变形菌纲(46.7 - 64.8%)是变形菌门中最主要的纲。还分离出了四株属于疣微菌门的菌株。系统发育分析表明,疣微菌门细菌菌株与盐单胞菌属或疣微菌属密切相关。