Hokkaido Agricultural Research Center, National Agriculture and Food Research Organization.
Microbes Environ. 2013;28(3):295-305. doi: 10.1264/jsme2.me13006. Epub 2013 Jun 8.
The bacterial community of a potato phytosphere at the flowering stage was examined using both culture-dependent and -independent methods. Tissues (leaves, stems, roots and tubers) were sampled from field-grown potato plants (cultivar Matilda), and the clone libraries of 16S rRNA genes and the isolate collections using R2A medium were constructed. By analyzing the combined data set of 16S rRNA gene sequences from both clone libraries and isolate collections, 82 genera from 8 phyla were found and 237 OTUs (≥97% identity) at species level were identified across the potato phytosphere. The statistical analyses of clone libraries suggested that stems harbor the lowest diversity among the tissues examined. The phylogenetic analyses revealed that the most dominant phylum was shown to be Proteobacteria for all tissues (62.0%-89.7% and 57.7%-72.9%, respectively), followed by Actinobacteria (5.0%-10.7% and 14.6%-39.4%, respectively). The results of principal coordinates analyses of both clone libraries and isolate collections indicated that distinct differences were observed between above- and below-ground tissues for bacterial community structures. The results also revealed that leaves harbored highly similar community structures to stems, while the tuber community was shown to be distinctly different from the stem and root communities.
采用培养依赖和非依赖的方法研究了花期马铃薯根际细菌群落。从田间生长的马铃薯植株（品种 Matilda）中采集组织（叶片、茎、根和块茎），构建了 16S rRNA 基因克隆文库和 R2A 培养基分离株集。通过分析克隆文库和分离株集的 16S rRNA 基因序列联合数据集，在马铃薯根际中发现了 8 个门的 82 个属和 237 个 OTUs（≥97%的同一性）。克隆文库的统计分析表明，在研究的组织中，茎的多样性最低。系统发育分析表明，所有组织中最主要的门都是变形菌门（分别为 62.0%-89.7%和 57.7%-72.9%），其次是放线菌门（分别为 5.0%-10.7%和 14.6%-39.4%）。克隆文库和分离株集主坐标分析的结果表明，地上和地下组织的细菌群落结构存在明显差异。结果还表明，叶片与茎具有高度相似的群落结构，而块茎群落与茎和根群落明显不同。
