Buscail L, Sénégas-Balas F, Balas D, Bouisson M, Bertrand C, Ribet A
INSERM U151, CHU Rangueil, Toulouse, France.
Pancreas. 1989;4(6):715-23. doi: 10.1097/00006676-198912000-00010.
This study was performed to assess the effects of misoprostol (M), a synthetic prostaglandin E1 analog, on experimental pancreatitis in rat. Pancreatitis was induced by ligation of the main pancreatic duct of 3-month-old male Wistar rats. Pancreatic lesions were observed at 6, 12, 24, 48, and 96 h after pancreatic duct ligation (PDL). A time of 48 h was chosen to evaluate M treatment. M was injected intraperitoneally (500 micrograms/kg every 4 h) between time 0 and 48 h after PDL. Stereological analysis was performed on light and electron microscopy. Total pancreatic amylase and chymotrypsin concentrations were determined. Four groups of five rats were studied: sham operated (SO), M without PDL (PG), duct ligation without M (DL), and duct ligation with M (DLPG). Edema, dedifferentiation of pancreatic acinar cells, and heterogeneous distribution of zymogen granule diameters observed after PDL were significantly decreased by M in the DLPG group. Enzyme concentrations were also decreased by M in the DLPG group. Enzyme concentrations were also decreased by M both in normal (PG) and duct ligated rats (DL). M has protective effects against pancreatic lesions induced by PDL. In this model, the protective effect of M may be due to a blockade of the autodigestive secretions of the pancreatic acinar cells.
本研究旨在评估合成前列腺素E1类似物米索前列醇（M）对大鼠实验性胰腺炎的影响。通过结扎3月龄雄性Wistar大鼠的主胰管诱导胰腺炎。在胰管结扎（PDL）后6、12、24、48和96小时观察胰腺病变。选择48小时来评估M治疗效果。在PDL后0至48小时之间腹腔注射M（每4小时500微克/千克）。对光镜和电镜进行体视学分析。测定胰腺总淀粉酶和胰凝乳蛋白酶浓度。研究了四组，每组五只大鼠：假手术组（SO）、未进行PDL的M组（PG）、未使用M的胰管结扎组（DL）和使用M的胰管结扎组（DLPG）。DLPG组中，PDL后观察到的水肿、胰腺腺泡细胞去分化以及酶原颗粒直径的不均匀分布通过M显著降低。DLPG组中M也降低了酶浓度。在正常（PG）大鼠和胰管结扎大鼠（DL）中，M均降低了酶浓度。M对PDL诱导的胰腺病变具有保护作用。在该模型中，M的保护作用可能是由于对胰腺腺泡细胞自消化性分泌的阻断。
