Crocket K V, Brackett K, Crocket A, Jacobson E D, Rao S S, Joffe S N
Eur Surg Res. 1984;16(5):265-73. doi: 10.1159/000128418.
Prostaglandin E1 (PGE1) was tested for cytoprotective activity against the development of experimental acute pancreatitis in the rat induced by the closed duodenal loop technique. Sham-operated, untreated and PGE1-treated pancreatic rats were investigated. All rats received an initial bolus of 3 ml 5% dextrose in normal saline (D5NS) via jugular catheter 30 min prior to surgery, and a continuous subcutaneous infusion of 35 ml D5NS over 24 h. Each treated rat received 10 micrograms/kg PGE1 in the initial bolus and a maintenance dosage of 10 micrograms/kg/h via the infusate. Serum amylase rose significantly in all pancreatic rats with no significant difference between treated and untreated. Pancreatic edema was more pronounced in PGE1-treated than in untreated rats. The ischemic and autolytic damage to acinar cells and vascular endothelial cells typical of untreated pancreatitis was delayed by PGE1. Mortality rates were unaffected by PGE1.
采用闭合十二指肠襻技术诱导大鼠实验性急性胰腺炎，检测前列腺素E1（PGE1）对其发展的细胞保护活性。对假手术、未治疗和PGE1治疗的胰腺大鼠进行了研究。所有大鼠在手术前30分钟经颈静脉导管接受3毫升5%葡萄糖生理盐水（D5NS）的初始推注，并在24小时内持续皮下输注35毫升D5NS。每只接受治疗的大鼠在初始推注中接受10微克/千克的PGE1，并通过输注液以10微克/千克/小时的剂量维持。所有胰腺大鼠的血清淀粉酶均显著升高，治疗组和未治疗组之间无显著差异。PGE1治疗的大鼠胰腺水肿比未治疗的大鼠更明显。PGE1延迟了未治疗胰腺炎典型的腺泡细胞和血管内皮细胞的缺血和自溶损伤。PGE1对死亡率无影响。
