溶液中臭氧的荧光测定法的优化。

Optimization of fluorometric assay for ozone in solution.

机构信息

Department of Analytical Biochemistry, School of Pharmacy, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.

出版信息

J Anal Methods Chem. 2014;2014:190937. doi: 10.1155/2014/190937. Epub 2014 Mar 24.

DOI:10.1155/2014/190937

PMID:24790771

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3982405/

Abstract

Ozone is widely used for sterilization, deodorization, and cleaning. However, the effects of ozone on human are not well understood, because there is currently no reliable assay method for ozone. Therefore the accurate measurement of ozone is important for its safe use. Here, we report optimized conditions for use with fluorescein derivative 1 (probe 1). Under optimum conditions, probe 1 reacted with ozone quantitatively and specifically to generate a fluorescent substance with an excitation maximum at 493 nm and an emission maximum at 523 nm. We conclude that this assay will be useful for quantifying ozone in solution.

摘要

臭氧被广泛应用于消毒、除臭和清洁。然而，人们对臭氧对人体的影响还不是很了解，因为目前还没有可靠的臭氧检测方法。因此，准确测量臭氧对于其安全使用非常重要。在这里，我们报告了使用荧光素衍生物 1（探针 1）的优化条件。在最佳条件下，探针 1 与臭氧定量且特异性地反应，生成激发最大波长为 493nm、发射最大波长为 523nm 的荧光物质。我们得出结论，该检测法将有助于定量检测溶液中的臭氧。