Csallany A S, Manwaring J D, Menken B Z
Environ Res. 1985 Aug;37(2):320-6. doi: 10.1016/0013-9351(85)90112-4.
Groups of ten female, weanling mice were fed a basal, vitamin E-deficient diet or a basal diet supplemented with RRR-alpha-tocopheryl acetate for 14 months. During the last month one group from each dietary regimen was exposed for 30-60 min/day to 1.5 ppm ozone (25 hr total ozone exposure) and the remaining groups to control ambient air. The liver and lung tissues were homogenized and extracted with 2:1 chloroform:methanol and water. The water-soluble and organic solvent-soluble fluorescent materials were separated on Sephadex G-25 and LH-20 columns, respectively. Excitation and emission wavelengths for the eluting fractions were determined by continuous emission scans from 250 to 600 nm for each excitation wavelength between 250 and 500 nm (in increments of 25 nm). Ozone exposure did not effect the concentration of any of the fluorescent materials examined in the lung, but it resulted in a significant increase in two of four water-soluble compounds in the liver with excitation wavelength maxima/emission wavelength maxima of 270 nm/310 nm and 275 nm/350 nm (smaller molecular weight material) suggesting in vivo lipid oxidation.
将十只雌性断乳小鼠分为一组，分别喂食基础维生素E缺乏饮食或添加了RRR-α-生育酚醋酸酯的基础饮食，持续14个月。在最后一个月，每种饮食方案中的一组小鼠每天暴露于1.5 ppm臭氧中30 - 60分钟（总臭氧暴露时间为25小时），其余组暴露于对照环境空气中。将肝脏和肺组织匀浆，并用2:1的氯仿：甲醇和水进行提取。水溶性和有机溶剂溶性荧光物质分别在Sephadex G - 25和LH - 20柱上分离。对于洗脱级分，在250至500 nm之间的每个激发波长（以25 nm为增量）下，通过从250至600 nm的连续发射扫描来确定激发和发射波长。臭氧暴露对肺中检测的任何荧光物质的浓度均无影响，但导致肝脏中四种水溶性化合物中的两种显著增加，其激发波长最大值/发射波长最大值分别为270 nm/310 nm和275 nm/350 nm（分子量较小的物质），表明体内脂质氧化。
