School of Pharmacy, China Pharmaceutical University, Nanjing 210009, China.
Department of Biomedical Engineering, School of Engineering, China Pharmaceutical University, Nanjing 210009, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2021 Mar 5;248:119192. doi: 10.1016/j.saa.2020.119192. Epub 2020 Nov 13.
Nowadays, ozone has been widely applied in industry and medical therapies. However, excessive exposure to ozone can lead to lung dysfunction and many respiratory symptoms. As a member of reactive oxygen species (ROS), ozone was also involved in various physiology and pathology process. Given the fact of this, the effective detection of ozone in the atmosphere and biological system is of vital significance. Herein, we reported a novel dicyanomethylene-4H-pyran (DCM)-based fluorescent probe DCM-O with butenyl being the recognition moiety for monitoring ozone. The probe displayed high selectivity towards ozone, and its response towards ozone could be completed within 5 min under the optimal condition. Besides, a good linear correlation was obtained between the ozone concentrations (0-50 μM) and the corresponding fluorescent intensity at 560 nm, and the limit of detection (LOD) was calculated to be 6.2 × 10 M. Moreover, the probe DCM-O showed low cytotoxicity and was successfully applied to detect ozone in live cells. Given all the merits, the probe DCM-O could function as a robust tool for researchers to investigate ozone-related diseases in the complex biological environment.
如今，臭氧已被广泛应用于工业和医疗领域。然而，过量的臭氧暴露会导致肺功能障碍和许多呼吸道症状。作为活性氧（ROS）的一种，臭氧也参与了各种生理和病理过程。鉴于此，有效检测大气和生物体系中的臭氧具有重要意义。在此，我们报道了一种基于二氰基乙烯基-4H-吡喃（DCM）的新型荧光探针 DCM-O，其中丁烯基作为识别部分，用于监测臭氧。该探针对臭氧具有高选择性，在最佳条件下，其对臭氧的响应可在 5 分钟内完成。此外，在 560nm 处，臭氧浓度（0-50μM）与相应荧光强度之间存在良好的线性关系，检测限（LOD）计算为 6.2×10-7 M。此外，探针 DCM-O 显示出较低的细胞毒性，并成功应用于活细胞中臭氧的检测。鉴于所有这些优点，探针 DCM-O 可以作为研究人员在复杂生物环境中研究与臭氧相关疾病的有力工具。
