Biophysics Department, Faculty of Science, Cairo University, 12613 Giza, Egypt.
Biophysics Department, Faculty of Science, Cairo University, 12613 Giza, Egypt.
Int J Pharm. 2014 Aug 15;470(1-2):1-7. doi: 10.1016/j.ijpharm.2014.04.067. Epub 2014 May 2.
The efficacy of the combined chemo-photothermal therapy, using a mesoporous silica-coated gold nanorods loaded DOX (pGNRs@mSiO2-DOX), was consistently tested both in vitro and in vivo. The prepared nanoparticles that were characterized using transmission electron microscopy (TEM), UV-vis absorption spectroscopy and zeta potential showed high doxorubicin loading capacity in addition to its pH-responsive release. The pGNRs@mSiO2-DOX photo-heat conversion characteristic found to be stable for several repeated NIR irradiated doses was tested in simulated body fluid. In vitro results showed that pGNRs@mSiO2-DOX causes a significant damage in breast cancer cell line MCF-7 compared to free DOX. Contrary to this, it showed low toxicity to human amnion wish cells compared to CTAB coated GNRs and free DOX. In vivo results showed that intravenous administration of pGNRs@mSiO2-DOX (1.7 mg/kg) markedly suppresses the growth of subcutaneous Ehrlich carcinoma in female Balb mice (p<0.0001). Consistently, histopathological examination revealed a complete loss of tumor cellular details for mice that received the combined treatment. Based on the obtained results, this passively targeted pGNRs@mSiO2-DOX could specifically deliver drug and excessive local heat to tumor sites achieving high combined therapeutic efficacy.
采用介孔硅包覆载阿霉素的金纳米棒（pGNRs@mSiO2-DOX）的联合化疗-光热疗法的疗效在体外和体内均得到了一致的测试。所制备的纳米粒子通过透射电子显微镜（TEM）、紫外-可见吸收光谱和zeta 电位进行了表征，具有高的阿霉素载药能力，此外还具有 pH 响应性释放。在模拟体液中测试了 pGNRs@mSiO2-DOX 的光热转换特性，发现其在多次重复近红外照射剂量下稳定。体外结果表明，与游离 DOX 相比，pGNRs@mSiO2-DOX 对乳腺癌细胞系 MCF-7 造成了显著的损伤。与之相反，与 CTAB 包覆的 GNRs 和游离 DOX 相比，它对人羊膜细胞的毒性较低。体内结果表明，静脉注射 pGNRs@mSiO2-DOX（1.7mg/kg）可显著抑制雌性 Balb 小鼠皮下 Ehrlich 癌的生长（p<0.0001）。一致地，组织病理学检查显示，接受联合治疗的小鼠的肿瘤细胞细节完全丧失。基于获得的结果，这种被动靶向的 pGNRs@mSiO2-DOX 可以将药物和过多的局部热量特异性递送至肿瘤部位，实现高的联合治疗效果。
