Johnson M D, Kamso-Pratt J, Federspiel C F, Whetsell W O
Department of Pathology, Vanderbilt University Medical Center, Nashville, TN 37232-2561.
Arch Pathol Lab Med. 1989 Nov;113(11):1263-70.
Cellular heterogeneity produced by non-Schwannian elements may distinguish neurofibromas from other Schwann cell neoplasma and contribute to a different tumor biology. The present study compared cell counts of mast cells, T and B lymphocytes, and macrophages in 32 neurofibromas with those in 27 schwannomas, 9 malignant nerve sheath tumors, and 17 traumatic neuromas. Immunohistochemical and histochemical analyses were performed on formalin-fixed, paraffin-embedded tissues using two monoclonal antibodies against B-lymphocyte epitopes (LN-1 and LN-2), one monoclonal antibody against T-lymphocyte epitopes (UCHL-1), one polyclonal antibody recognizing alpha 1-antichymotrypsin (ACT), a macrophage/histiocytemarker, and toluidine blue O stains. Neurofibromas contained relatively high concentrations of mast cells significantly greater than the concentrations in other neoplastic or reactive nerve sheath tumors. Most neurofibromas also displayed moderate concentrations of LN-2 immunoreactive cells, similar to the concentrations in traumatic neuromas and not statistically different from cell counts in other tumor types. Limited, variable LN-1 and UCHL-1 immunoreactive infiltrates were detected in neurofibromas and some peripheral schwannomas. Rare or moderate ACT immunoreactivity was detected in the majority of neurofibromas, in contrast with the absence, or rare appearance, of ACT immunostaining in cranial and peripheral nerve schwannomas and moderate numbers of immunoreactive cells in many malignant nerve sheath tumors. Mast cells are an important cellular marker of neurofibromas and may participate in the pathogenesis of these neoplasms.
非施万细胞成分产生的细胞异质性可能使神经纤维瘤有别于其他施万细胞瘤，并导致不同的肿瘤生物学特性。本研究比较了32例神经纤维瘤中肥大细胞、T淋巴细胞、B淋巴细胞和巨噬细胞的细胞计数与27例神经鞘瘤、9例恶性神经鞘膜瘤和17例创伤性神经瘤中的细胞计数。使用两种抗B淋巴细胞表位的单克隆抗体（LN-1和LN-2）、一种抗T淋巴细胞表位的单克隆抗体（UCHL-1）、一种识别α1-抗糜蛋白酶（ACT）的多克隆抗体（一种巨噬细胞/组织细胞标志物）以及甲苯胺蓝O染色，对福尔马林固定、石蜡包埋的组织进行免疫组织化学和组织化学分析。神经纤维瘤中肥大细胞浓度相对较高，显著高于其他肿瘤性或反应性神经鞘瘤中的浓度。大多数神经纤维瘤还显示出中等浓度的LN-2免疫反应性细胞，类似于创伤性神经瘤中的浓度，与其他肿瘤类型的细胞计数无统计学差异。在神经纤维瘤和一些周围神经鞘瘤中检测到有限的、可变的LN-1和UCHL-1免疫反应性浸润。大多数神经纤维瘤中检测到罕见或中等程度的ACT免疫反应性，与之形成对比的是，颅神经和周围神经鞘瘤中ACT免疫染色缺失或罕见出现，而许多恶性神经鞘膜瘤中有中等数量的免疫反应性细胞。肥大细胞是神经纤维瘤的重要细胞标志物，可能参与这些肿瘤的发病机制。
