Schaafsma M R, Fibbe W E, Van Damme J, Duinkerken N, Ralph P, Kaushansky K, Altrock B W, Willemze R, Falkenburg J H
Department of Hematology, University Medical Center, Leiden, The Netherlands.
Blood. 1989 Dec;74(8):2619-23.
Interleukin-6 (IL-6) is a multifunctional cytokine that plays a role in regulation of hematopoiesis. Because IL-6 is coinduced with colony-stimulating factors (CSFs) by various cell types in response to stimulation with IL-1, we investigated whether IL-6 is involved in the IL-1-induced production of CSF by human bone marrow (BM) cells in long-term culture or human fibroblasts. We showed that IL-6 does not induce CSF production by these cells. Neither addition of exogenous IL-6 nor neutralization of endogenous production of IL-6 by an anti-IL-6 monoclonal antibody (MoAb) diminished the IL-1-induced colony-stimulating activity (CSA), indicating that IL-6 did not act synergistically with IL-1. Finally, IL-6 did not influence the kinetics of IL-1-induced CSA production by human fibroblasts. We conclude that IL-6, either alone or in combination with IL-1, does not induce CSF production by human BM stromal cells or fibroblasts.
白细胞介素-6（IL-6）是一种多功能细胞因子，在造血调节中发挥作用。由于IL-6在多种细胞类型受到IL-1刺激时与集落刺激因子（CSF）共同诱导产生，我们研究了IL-6是否参与长期培养的人骨髓（BM）细胞或人成纤维细胞中IL-1诱导的CSF产生。我们发现IL-6不会诱导这些细胞产生CSF。添加外源性IL-6或用抗IL-6单克隆抗体（MoAb）中和内源性IL-6产生均未降低IL-1诱导的集落刺激活性（CSA），这表明IL-6不会与IL-1协同作用。最后，IL-6不影响人成纤维细胞中IL-1诱导的CSA产生动力学。我们得出结论，IL-6单独或与IL-1联合使用，均不会诱导人BM基质细胞或成纤维细胞产生CSF。
