Suzuki A, Takahashi T, Okuno Y, Tsuyuoka R, Fukumoto M, Nakamura K, Imura H
Second Department of Internal Medicine, Kyoto University School of Medicine, Japan.
Br J Cancer. 1992 Apr;65(4):515-8. doi: 10.1038/bjc.1992.106.
We previously demonstrated that colony stimulating factor (CSF)-producing cell lines co-produce interleukin-1 (IL-1) and IL-6 in addition to CSFs. In the present study, we examined the role of IL-1 production in three human tumour cell lines producing granulocyte (G)-CSF, IL-1 and IL-6. Addition of anti-human IL-1 alpha antiserum to the culture caused a 90-62% reduction of G-CSF and a 85-44% reduction of IL-6 production, respectively, as evaluated by enzyme immunoassay in all three cell lines. The decrease of G-CSF and IL-6 production by the anti-IL-1 alpha antiserum was also confirmed at the level of mRNA expression. The anti-IL-1 alpha antiserum did not affect the growth of these cell lines. Excess recombinant IL-1 alpha exogenously added to the culture enhanced G-CSF and IL-6 production in all three cell lines. However, IL-1 alpha had little effect on the growth of these three cell lines. Neither anti-IL-6 nor anti-G-CSF antibodies affected the production of the other cytokines. These results indicate that IL-1 alpha regulates G-CSF and IL-6 production in these tumour cell lines, and suggest that the IL-1 production plays an important role in CSF-producing tumours.
我们先前证明，产生集落刺激因子（CSF）的细胞系除了产生CSF外，还共同产生白细胞介素-1（IL-1）和IL-6。在本研究中，我们检测了IL-1产生在三种产生粒细胞（G）-CSF、IL-1和IL-6的人肿瘤细胞系中的作用。在所有三种细胞系中，通过酶免疫测定法评估，向培养物中添加抗人IL-1α抗血清分别导致G-CSF产生减少90 - 62%，IL-6产生减少85 - 44%。抗IL-1α抗血清对G-CSF和IL-6产生的减少在mRNA表达水平也得到了证实。抗IL-1α抗血清不影响这些细胞系的生长。向培养物中外源添加过量的重组IL-1α可增强所有三种细胞系中G-CSF和IL-6的产生。然而，IL-1α对这三种细胞系的生长几乎没有影响。抗IL-6和抗G-CSF抗体均不影响其他细胞因子的产生。这些结果表明，IL-1α调节这些肿瘤细胞系中G-CSF和IL-6的产生，并提示IL-1的产生在产生CSF的肿瘤中起重要作用。
