Tremblay M, Wainberg M A
Lady Davis Institute for Medical Research of the Sir Mortimer B. Davis-Jewish General Hospital, Montreal, Quebec, Canada.
Cancer Lett. 1989 Nov 30;48(2):95-103. doi: 10.1016/0304-3835(89)90043-8.
We have examined 3 different EBV-carrying B cell lines, in terms of ability to be super-infected by the human immunodeficiency virus (HIV-1), and have followed these lines, after infection by HIV-1, over a period of 3 months. We found significant variation among different HIV-1 strains in terms of the multiplicity of infection required to initiate infection in these EBV-positive cell lines. Persistent infection by HIV-1 in the absence of detectable cytopathic effects could be demonstrated, as evaluated by a variety of techniques, including reverse transcriptase assay and immunofluorescence. The results indicate also that all of these cell lines produced progeny HIV-1 intermittently, with large amounts of virus production on some days but not others. In contrast, they were all able to continuously express p24.
我们检测了3种不同的携带EB病毒（EBV）的B细胞系感染人类免疫缺陷病毒1型（HIV-1）的能力，并在这些细胞系被HIV-1感染后，对其进行了为期3个月的跟踪观察。我们发现，在这些EBV阳性细胞系中引发感染所需的感染复数方面，不同的HIV-1毒株存在显著差异。通过包括逆转录酶测定和免疫荧光在内的多种技术评估，可证明HIV-1在无明显细胞病变效应的情况下持续感染。结果还表明，所有这些细胞系均间歇性产生HIV-1子代，某些日子产生大量病毒，而其他日子则不然。相比之下，它们均能够持续表达p24。
