组织培养上清液中HIV-1活性的定量：培养条件对合胞体检测及病毒产生的影响。
Quantitation of HIV-1 activity in tissue culture supernatants: effects of culture condition on syncytial assays and virus production.
作者信息
Spickett G, Beattie R E, Bountiff L, Dalgleish A G, Webster A D
机构信息
Department of Immunological Medicine, Clinical Research Centre, Harrow, Oxford, U.K.
出版信息
J Virol Methods. 1989 Apr-May;24(1-2):67-76. doi: 10.1016/0166-0934(89)90008-6.
PMID:2474561
Abstract
We have compared the infectivity titres, reverse transcriptase levels and antigen titres in the supernatants from persistently infected cell lines that produce a variety of HIV-1 isolates. We found a poor correlation between the different assays, and a variability in viral activity dependent on culture medium.
摘要
我们比较了来自产生多种HIV-1分离株的持续感染细胞系的上清液中的感染性滴度、逆转录酶水平和抗原滴度。我们发现不同检测方法之间的相关性较差，并且病毒活性存在因培养基而异的情况。
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