神经元功能标记的新纪元：活性依赖启动子崭露头角。
A new era for functional labeling of neurons: activity-dependent promoters have come of age.
机构信息
Department of Neurochemistry, Graduate School of Medicine, The University of Tokyo Tokyo, Japan.
Department of Neurochemistry, Graduate School of Medicine, The University of Tokyo Tokyo, Japan ; Core Research for Evolutionary Science and Technology, Japan Science and Technology Agency Saitama, Japan.
出版信息
Front Neural Circuits. 2014 Apr 23;8:37. doi: 10.3389/fncir.2014.00037. eCollection 2014.
Genetic labeling of neurons with a specific response feature is an emerging technology for precise dissection of brain circuits that are functionally heterogeneous at the single-cell level. While immediate early gene mapping has been widely used for decades to identify brain regions which are activated by external stimuli, recent characterization of the promoter and enhancer elements responsible for neuronal activity-dependent transcription have opened new avenues for live imaging of active neurons. Indeed, these advancements provided the basis for a growing repertoire of novel experiments to address the role of active neuronal networks in cognitive behaviors. In this review, we summarize the current literature on the usage and development of activity-dependent promoters and discuss the future directions of this expanding new field.
用具有特定反应特征的神经元进行遗传标记是一种新兴技术，可以精确剖析在单细胞水平上功能异质的大脑回路。虽然即时早期基因图谱已被广泛应用数十年，用于识别受外部刺激激活的脑区，但最近对负责神经元活动依赖性转录的启动子和增强子元件的特征描述，为活性神经元的实时成像开辟了新途径。事实上，这些进展为一系列新的实验提供了基础，这些实验旨在解决活性神经元网络在认知行为中的作用。在这篇综述中，我们总结了关于活性依赖性启动子的使用和发展的现有文献，并讨论了这个不断扩展的新领域的未来方向。
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