Department of Medicine, Morehouse School of Medicine, Atlanta, GA 30310, USA.
Comprehensive Cancer Center University of Alabama at Birmingham, Birmingham, AL 35249, USA.
Biomed Res Int. 2014;2014:585083. doi: 10.1155/2014/585083. Epub 2014 Mar 27.
Serous subtype of ovarian cancer is considered to originate from fallopian epithelium mucosa that has been exposed to physiological changes resulting from ovulation. Ovulation influences an increased in inflammation of epithelial ovarian cells as results of constant exposure of cells to ROS. The imbalance between ROS and antioxidant capacities, as well as a disruption of redox signaling, causes a wide range of damage to DNA, proteins, and lipids. This study applied spectrophotometric, dinitrophenylhydrazone (DNPH) assay, two-dimensional gel electrophoresis, and Western blot analyses to assess the levels of oxidatively modified proteins in 100 primary serous epithelial ovarian carcinoma and normal/surrounding tissues. These samples were obtained from 56 Caucasian and 44 African-American patients within the age range of 61 ± 10 years. Analyses showed that the levels of reactive protein carbonyl groups increased as stages progressed to malignancy. Additionally, the levels of protein carbonyls in serous ovarian carcinoma among African Americans are 40% (P < 0.05) higher relative to Caucasian at similar advanced stages. Results suggest that oxidative stress is involved in the modification of carbonyl protein groups, leading to increased aggressiveness of epithelial ovarian tumors and may contribute to the disease's invasiveness among African Americans.
浆液性卵巢癌被认为起源于暴露于排卵引起的生理变化的输卵管上皮黏膜。排卵会导致上皮性卵巢细胞的炎症增加，这是由于细胞持续暴露于 ROS 所致。ROS 和抗氧化能力之间的失衡以及氧化还原信号的中断，会导致 DNA、蛋白质和脂质受到广泛的损伤。本研究应用分光光度法、二硝基苯肼（DNPH）测定法、二维凝胶电泳和 Western blot 分析来评估 100 例原发性浆液性上皮性卵巢癌和正常/周围组织中氧化修饰蛋白的水平。这些样本来自 56 名高加索人和 44 名非裔美国人患者，年龄在 61 ± 10 岁之间。分析表明，随着疾病向恶性进展，反应性蛋白羰基水平升高。此外，在相似的晚期阶段，非裔美国人浆液性卵巢癌中的蛋白羰基水平比高加索人高 40%（P < 0.05）。结果表明，氧化应激参与了羰基蛋白基团的修饰，导致上皮性卵巢肿瘤的侵袭性增加，并可能导致非裔美国人中该疾病的侵袭性。
