Wang N, Zhang S, Zhang A F, Yang Z Y, Li X G
Department of Neurobiology and Beijing Institute for Neuroscience, Capital Medical University, Beijing, China.
China National Center of Biomedical Analysis, Beijing, China.
Spinal Cord. 2014 Jul;52(7):517-23. doi: 10.1038/sc.2014.54. Epub 2014 May 6.
Currently, effective therapeutic strategy for spinal cord injury (SCI) is not clinically available. To establish a better method that may help repair the injured spinal cord, sodium hyaluronate-ciliary neurotrophic factor (CNTF) gelatinous particles were generated.
A segment of spinal cord tissue was excised to form a 2.5-mm-long cavity at thoracic level in an adult rat, and sodium hyaluronate-CNTF gelatinous particles were implanted into the lesion cavity. The recovery of the injured spinal cord was evaluated by immunohistochemistry, nerve tracing, electrophysiological test and Basso-Beattie-Bresnahan locomotor rating scale.
Open-field locomotion of the sodium hyaluronate-CNTF rats was significantly enhanced up to 12 weeks postoperation. Together with the evidence of enhanced cortical motor evoked potentials and cortical somatosensory evoked potentials in the sodium hyaluronate-CNTF group, these findings suggested a powerful functional recovery component. Immunohistochemical analyses suggested that the functional recovery might be attributable partly to an increase in axonal regrowth as well as in replenishment of β-tubulin-III-positive neuron-like cells.
Sodium hyaluronate-CNTF gelatinous particles may provide an effective method for treating SCI.
目前，临床上尚无治疗脊髓损伤（SCI）的有效策略。为建立一种可能有助于修复受损脊髓的更好方法，制备了透明质酸钠-睫状神经营养因子（CNTF）凝胶颗粒。
切除成年大鼠胸段脊髓组织，形成一个2.5毫米长的空洞，并将透明质酸钠-CNTF凝胶颗粒植入损伤空洞。通过免疫组织化学、神经追踪、电生理测试和Basso-Beattie-Bresnahan运动评分量表评估受损脊髓的恢复情况。
术后12周，透明质酸钠-CNTF组大鼠的旷场运动能力显著增强。结合透明质酸钠-CNTF组皮质运动诱发电位和皮质体感诱发电位增强的证据，这些结果提示了强大的功能恢复成分。免疫组织化学分析表明，功能恢复可能部分归因于轴突再生增加以及β-微管蛋白III阳性神经元样细胞的补充。
透明质酸钠-CNTF凝胶颗粒可能为治疗脊髓损伤提供一种有效方法。
